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유전체 교정 연구단
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Off-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing

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dc.contributor.authorAnnie Kim-
dc.contributor.authorKun-Gu Lee-
dc.contributor.authorYeongbeen Kwon-
dc.contributor.authorKang-In Lee-
dc.contributor.authorHeung-Mo Yang-
dc.contributor.authorOmer Habib-
dc.contributor.authorJihun Kim-
dc.contributor.authorSang-Tae Kim-
dc.contributor.authorSung Joo Kim-
dc.contributor.authorJin-Soo Kim-
dc.contributor.authorDong-Youn Hwang-
dc.date.accessioned2021-07-05T02:30:03Z-
dc.date.accessioned2021-07-05T02:30:03Z-
dc.date.available2021-07-05T02:30:03Z-
dc.date.available2021-07-05T02:30:03Z-
dc.date.created2021-02-23-
dc.date.issued2021-06-
dc.identifier.issn2629-3269-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/9837-
dc.description.abstractHuman embryonic stem cells (hESCs) hold promise in regenerative medicine but allogeneic immune rejections caused by highly polymorphic human leukocyte antigens (HLAs) remain a barrier to their clinical applications. Here, we used a CRISPR/Cas9-mediated HLA-editing strategy to generate a variety of HLA homozygous-like hESC lines from pre-established hESC lines. We edited four pre-established HLA-heterozygous hESC lines and created a mini library of 14 HLA-edited hESC lines in which single HLA-A and HLA-B alleles and both HLA-DR alleles are disrupted. The HLA-edited hESC derivatives elicited both low T cell- and low NK cell-mediated immune responses. Our library would cover about 40% of the Asian-Pacific population. We estimate that HLA-editing of only 19 pre-established hESC lines would give rise to 46 different hESC lines to cover 90% of the Asian-Pacific population. This study offers an opportunity to generate an off-the-shelf HLA-compatible hESC bank, available for immune-compatible cell transplantation, without embryo destruction. Graphical-
dc.description.uri1-
dc.language영어-
dc.publisherSPRINGER-
dc.titleOff-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000606409400001-
dc.identifier.scopusid2-s2.0-85098973871-
dc.identifier.rimsid74627-
dc.contributor.affiliatedAuthorAnnie Kim-
dc.contributor.affiliatedAuthorSang-Tae Kim-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1007/s12015-020-10113-7-
dc.identifier.bibliographicCitationSTEM CELL REVIEWS AND REPORTS, v.17, no.3, pp.1053 - 1067-
dc.citation.titleSTEM CELL REVIEWS AND REPORTS-
dc.citation.volume17-
dc.citation.number3-
dc.citation.startPage1053-
dc.citation.endPage1067-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryCell & Tissue Engineering-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.relation.journalWebOfScienceCategoryMedicine, Research & Experimental-
dc.subject.keywordPlusMESSENGER-RNA DECAY-
dc.subject.keywordPlusCLASS-I-
dc.subject.keywordPlusSOMATIC-CELLS-
dc.subject.keywordPlusHLA-
dc.subject.keywordPlusANTIGEN-
dc.subject.keywordPlusLINES-
dc.subject.keywordPlusREJECTION-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusPROLIFERATION-
dc.subject.keywordPlusFREQUENCIES-
dc.subject.keywordAuthorImmune-compatible hESC banking-
dc.subject.keywordAuthorHuman embryonic stem cells-
dc.subject.keywordAuthorHLA-editing-
dc.subject.keywordAuthorCRISPR-
dc.subject.keywordAuthorCas9-
dc.subject.keywordAuthorHLA homozygous-like hESCs-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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