BROWSE

Related Scientist

rna's photo.

rna
rna연구단
more info

ITEM VIEW & DOWNLOAD

Quantification of purified endogenous miRNAs with high sensitivity and specificity

DC Field Value Language
dc.contributor.authorShin, Soochul-
dc.contributor.authorYoonseok Jung-
dc.contributor.authorUhm, Heesoo-
dc.contributor.authorSong, Minseok-
dc.contributor.authorSoomin Son-
dc.contributor.authorGoo, Jiyoung-
dc.contributor.authorJeong, Cherlhyun-
dc.contributor.authorSong, Ji-Joon-
dc.contributor.authorV.Narry Kim-
dc.contributor.authorHohng, Sungchul-
dc.date.accessioned2021-01-06T01:50:02Z-
dc.date.accessioned2021-01-06T01:50:02Z-
dc.date.available2021-01-06T01:50:02Z-
dc.date.available2021-01-06T01:50:02Z-
dc.date.created2020-12-09-
dc.date.issued2020-12-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/8989-
dc.description.abstractMicroRNAs (miRNAs) are short (19-24 nt) non-coding RNAs that suppress the expression of protein coding genes at the post-transcriptional level. Differential expression profiles of miRNAs across a range of diseases have emerged as powerful biomarkers, making a reliable yet rapid profiling technique for miRNAs potentially essential in clinics. Here, we report an amplification-free multi-color single-molecule imaging technique that can profile purified endogenous miRNAs with high sensitivity, specificity, and reliability. Compared to previously reported techniques, our technique can discriminate single base mismatches and single-nucleotide 3-tailing with low false positive rates regardless of their positions on miRNA. By preloading probes in Thermus thermophilus Argonaute (TtAgo), miRNAs detection speed is accelerated by more than 20 times. Finally, by utilizing the well-conserved linearity between single-molecule spot numbers and the target miRNA concentrations, the absolute average copy numbers of endogenous miRNA species in a single cell can be estimated. Thus our technique, Ago-FISH (Argonaute-based Fluorescence In Situ Hybridization), provides a reliable way to accurately profile various endogenous miRNAs on a single miRNA sensing chip.-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE RESEARCH-
dc.titleQuantification of purified endogenous miRNAs with high sensitivity and specificity-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000596476900010-
dc.identifier.scopusid2-s2.0-85096784225-
dc.identifier.rimsid73950-
dc.contributor.affiliatedAuthorYoonseok Jung-
dc.contributor.affiliatedAuthorSoomin Son-
dc.contributor.affiliatedAuthorV.Narry Kim-
dc.identifier.doi10.1038/s41467-020-19865-9-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, v.11, no.1, pp.1 - 8-
dc.citation.titleNATURE COMMUNICATIONS-
dc.citation.volume11-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage8-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusDNA-PAINT-
dc.subject.keywordPlusMICRORNA-
dc.subject.keywordPlusURIDYLATION-
dc.subject.keywordPlusBIOGENESIS-
dc.subject.keywordPlusRNAS-
dc.subject.keywordAuthorDNA-PAINT-
dc.subject.keywordAuthorMICRORNA-
dc.subject.keywordAuthorURIDYLATION-
dc.subject.keywordAuthorBIOGENESIS-
dc.subject.keywordAuthorRNAS-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse