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The Architecture of SARS-CoV-2 TranscriptomeHighly Cited Paper

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dc.contributor.authorDongwan Kim-
dc.contributor.authorJoo-Yeon Lee-
dc.contributor.authorJeong-Sun Yang-
dc.contributor.authorJun Won Kim-
dc.contributor.authorV. Narry Kim-
dc.contributor.authorHyeshik Chang-
dc.date.accessioned2020-12-22T06:33:47Z-
dc.date.accessioned2020-12-22T06:33:48Z-
dc.date.available2020-12-22T06:33:47Z-
dc.date.available2020-12-22T06:33:48Z-
dc.date.created2020-06-29-
dc.date.issued2020-05-
dc.identifier.issn0092-8674-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/8628-
dc.description.abstract© 2020 Elsevier Inc. The SARS-CoV-2 transcriptome and epitranscriptome reveal a complex array of canonical and non-canonical viral transcripts with RNA modifications. © 2020 Elsevier Inc.SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3′ tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2-
dc.description.uri1-
dc.language영어-
dc.publisherCELL PRESS-
dc.subjectcoronavirus-
dc.subjectCOVID-19-
dc.subjectdirect RNA sequencing-
dc.subjectdiscontinuous transcription-
dc.subjectepitranscriptome-
dc.subjectnanopore-
dc.subjectpoly(A) tail-
dc.subjectRNA modification-
dc.subjectSARS-CoV-2-
dc.subjecttranscriptome-
dc.titleThe Architecture of SARS-CoV-2 Transcriptome-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000533623900016-
dc.identifier.scopusid2-s2.0-85083509190-
dc.identifier.rimsid72012-
dc.contributor.affiliatedAuthorDongwan Kim-
dc.contributor.affiliatedAuthorV. Narry Kim-
dc.contributor.affiliatedAuthorHyeshik Chang-
dc.identifier.doi10.1016/j.cell.2020.04.011-
dc.identifier.bibliographicCitationCELL, v.181, no.4, pp.914 - 921-
dc.citation.titleCELL-
dc.citation.volume181-
dc.citation.number4-
dc.citation.startPage914-
dc.citation.endPage921-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusRNA-SYNTHESIS-
dc.subject.keywordPlusCORONAVIRUS-
dc.subject.keywordPlusN6-METHYLADENOSINE-
dc.subject.keywordPlusGENOMES-
dc.subject.keywordAuthorcoronavirus-
dc.subject.keywordAuthorCOVID-19-
dc.subject.keywordAuthordirect RNA sequencing-
dc.subject.keywordAuthordiscontinuous transcription-
dc.subject.keywordAuthorepitranscriptome-
dc.subject.keywordAuthornanopore-
dc.subject.keywordAuthorpoly(A) tail-
dc.subject.keywordAuthorRNA modification-
dc.subject.keywordAuthorSARS-CoV-2-
dc.subject.keywordAuthortranscriptome-
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Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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