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Graphene oxide-based molecular diagnostic biosensor for simultaneous detection of Zika and dengue viruses

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dc.contributor.authorJi-Seon Lee-
dc.contributor.authorKim, Jungho-
dc.contributor.authorHojeong Shin-
dc.contributor.authorDal-Hee Min-
dc.date.accessioned2020-12-22T06:27:11Z-
dc.date.accessioned2020-12-22T06:27:11Z-
dc.date.available2020-12-22T06:27:11Z-
dc.date.available2020-12-22T06:27:11Z-
dc.date.created2020-09-09-
dc.date.issued2020-10-
dc.identifier.issn2053-1583-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/8471-
dc.description.abstract© 2020 IOP Publishing Ltd. The world's tropical and sub-tropical regions are at risk for Zika and dengue virus (ZIKV, DENV) spread. Co-infection between ZIKV and DENV and repeated DENV infections of different serotypes increase the disease severity by impairing immune responses. Therefore, to avoid high risks of virus infection and achieve effective clinical management, development of diagnostic sensor for rapid and accurate identification of each virus is very important. Herein, we report a graphene oxide-based multiplexed detection strategy to make the differential diagnosis of Zika and dengue viruses by targeting virus nucleic acid. The fluorometric detection strategy is integrated with loop-mediated isothermal amplification assay to further increase sensitivity. In the presence of target virus, fluorescent PNA probes recognize the single-stranded loops of the cauliflower-like-shaped virus amplicon in a sequence-specific manner. Meanwhile, graphene oxide functions to distinguish the valid fluorescence signal as a selective fluorescence quencher for single-stranded PNA probes. The present strategy allows multiplexed detection of closely related viruses for specific discrimination of the virus nucleic acids with simplified molecular diagnostic steps, without requiring any nucleic acid isolation step-
dc.description.uri1-
dc.language영어-
dc.publisherIOP PUBLISHING LTD-
dc.subjectdengue virus-
dc.subjectgraphene oxide-
dc.subjectisothermal amplification-
dc.subjectmolecular diagnostics-
dc.subjectmultiplexed detection-
dc.subjectpeptide nucleic acid-
dc.subjectZika virus-
dc.titleGraphene oxide-based molecular diagnostic biosensor for simultaneous detection of Zika and dengue viruses-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000553267900001-
dc.identifier.scopusid2-s2.0-85089778764-
dc.identifier.rimsid72942-
dc.contributor.affiliatedAuthorJi-Seon Lee-
dc.contributor.affiliatedAuthorHojeong Shin-
dc.contributor.affiliatedAuthorDal-Hee Min-
dc.identifier.doi10.1088/2053-1583/ab9a64-
dc.identifier.bibliographicCitation2D MATERIALS, v.7, no.4, pp.044001-
dc.citation.title2D MATERIALS-
dc.citation.volume7-
dc.citation.number4-
dc.citation.startPage044001-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusMEDIATED ISOTHERMAL AMPLIFICATION-
dc.subject.keywordPlusNUCLEIC-ACID-
dc.subject.keywordPlusRAPID DETECTION-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusPNA-
dc.subject.keywordPlusPLATFORM-
dc.subject.keywordPlusDISEASE-
dc.subject.keywordAuthordengue virus-
dc.subject.keywordAuthorgraphene oxide-
dc.subject.keywordAuthorisothermal amplification-
dc.subject.keywordAuthormolecular diagnostics-
dc.subject.keywordAuthormultiplexed detection-
dc.subject.keywordAuthorpeptide nucleic acid-
dc.subject.keywordAuthorZika virus-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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