BROWSE

Related Scientist

rna's photo.

rna
rna연구단
more info

ITEM VIEW & DOWNLOAD

BSA as additive: A simple strategy for practical applications of PNA in bioanalysis

DC Field Value Language
dc.contributor.authorJieon Lee-
dc.contributor.authorIl-Soo Park-
dc.contributor.authorKim Henna-
dc.contributor.authorJae-Sung Woo-
dc.contributor.authorChoi Byong-Seok-
dc.contributor.authorDal-Hee Min-
dc.date.available2015-04-20T05:10:19Z-
dc.date.created2015-03-16-
dc.date.issued2015-07-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/819-
dc.description.abstractApplication of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds-
dc.description.uri1-
dc.language영어-
dc.publisherELSEVIER ADVANCED TECHNOLOGY-
dc.subjectBioanalysis, Bovine serumalbumin, Graphene oxide, Peptide nucleicacid, Signal enhancement-
dc.titleBSA as additive: A simple strategy for practical applications of PNA in bioanalysis-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000356642900022-
dc.identifier.scopusid2-s2.0-84923637597-
dc.identifier.rimsid18426ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorJieon Lee-
dc.contributor.affiliatedAuthorIl-Soo Park-
dc.contributor.affiliatedAuthorJae-Sung Woo-
dc.contributor.affiliatedAuthorDal-Hee Min-
dc.identifier.doi10.1016/j.bios.2015.02.030-
dc.identifier.bibliographicCitationBIOSENSORS & BIOELECTRONICS, v.69, pp.167 - 173-
dc.citation.titleBIOSENSORS & BIOELECTRONICS-
dc.citation.volume69-
dc.citation.startPage167-
dc.citation.endPage173-
dc.date.scptcdate2018-10-01-
dc.description.wostc13-
dc.description.scptc13-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusPEPTIDE NUCLEIC-ACID-
dc.subject.keywordPlusBOVINE SERUM-ALBUMIN-
dc.subject.keywordPlusGRAPHENE OXIDE-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusSOLUBILITY-
dc.subject.keywordPlusASSAY-
dc.subject.keywordPlusHYBRIDIZATION-
dc.subject.keywordPlusOLIGOMERS-
dc.subject.keywordPlusPLATFORM-
dc.subject.keywordPlusBINDING-
dc.subject.keywordAuthorBioanalysis-
dc.subject.keywordAuthorBovine serum albumin-
dc.subject.keywordAuthorGraphene oxide-
dc.subject.keywordAuthorPeptide nucleic acid-
dc.subject.keywordAuthorSignal enhancement-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
BSA as additive.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse