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유전체교정연구단
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Genome-wide specificity of dCpf1 cytidine base editors

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dc.contributor.authorDaesik Kim-
dc.contributor.authorKayeong Lim-
dc.contributor.authorDa-eun Kim-
dc.contributor.authorJin-Soo Kim-
dc.date.accessioned2020-12-22T02:48:54Z-
dc.date.accessioned2020-12-22T02:48:54Z-
dc.date.available2020-12-22T02:48:54Z-
dc.date.available2020-12-22T02:48:54Z-
dc.date.created2020-09-09-
dc.date.issued2020-08-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/7706-
dc.description.abstract© 2020 The Author(s). Cpf1-linked base editors broaden the targeting scope of programmable cytidine deaminases by recognizing thymidine-rich protospacer-adjacent motifs (PAM) without inducing DNA double-strand breaks (DSBs). Here we present an unbiased in vitro method for identifying genome-wide off-target sites of Cpf1 base editors via whole genome sequencing. First, we treat human genomic DNA with dLbCpf1-BE ribonucleoprotein (RNP) complexes, which convert C-to-U at on-target and off-target sites and, then, with a mixture of E. coli uracil DNA glycosylase (UDG) and DNA glycosylase-lyase Endonuclease VIII, which removes uracil and produces single-strand breaks (SSBs) in vitro. Whole-genome sequencing of the resulting digested genome (Digenome-seq) reveals that, on average, dLbCpf1-BE induces 12 SSBs in vitro per crRNA in the human genome. Off-target sites with an editing frequency as low as 0.1% are successfully identified by this modified Digenome-seq method, demonstrating its high sensitivity. dLbCpf1-BEs and LbCpf1 nucleases often recognize different off-target sites, calling for independent analysis of each tool-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectDNA-
dc.titleGenome-wide specificity of dCpf1 cytidine base editors-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000564248600010-
dc.identifier.scopusid2-s2.0-85089368744-
dc.identifier.rimsid72909-
dc.contributor.affiliatedAuthorDaesik Kim-
dc.contributor.affiliatedAuthorKayeong Lim-
dc.contributor.affiliatedAuthorDa-eun Kim-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1038/s41467-020-17889-9-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, v.11, no.1, pp.4072-
dc.citation.titleNATURE COMMUNICATIONS-
dc.citation.volume11-
dc.citation.number1-
dc.citation.startPage4072-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusDNA-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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