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Lee, Seong Gyu
단백질 커뮤니케이션 그룹
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ClpL is a functionally active tetradecameric AAA plus chaperone, distinct from hexameric/dodecameric ones

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Title
ClpL is a functionally active tetradecameric AAA plus chaperone, distinct from hexameric/dodecameric ones
Author(s)
Gyuhee Kim; Seong-Gyu Lee; Seungsu Han; Jaeeun Jung; Hyeong Seop Jeong; Jae-kyung Hyun; Dong-Kwon Rhee; Ho Min Kim; Sangho Lee
Subject
chaperone, ; ClpL, ; Hsp100, ; S, ; pneumoniae, ; tetradecamer
Publication Date
2020-11
Journal
FASEB JOURNAL, v.34, no.11, pp.14353 - 14370
Publisher
FEDERATION AMER SOC EXP BIOL
Abstract
© 2020 Federation of American Societies for Experimental Biology. AAA+ (ATPases associated with diverse cellular activities) chaperones are involved in a plethora of cellular activities to ensure protein homeostasis. The function of AAA+ chaperones is mostly modulated by their hexameric/dodecameric quaternary structures. Here we report the structural and biochemical characterizations of a tetradecameric AAA+ chaperone, ClpL fromStreptococcus pneumoniae. ClpL exists as a tetradecamer in solution in the presence of ATP. The cryo-EM structure of ClpL at 4.5 angstrom resolution reveals a striking tetradecameric arrangement. Solution structures of ClpL derived from small-angle X-ray scattering data suggest that the tetradecameric ClpL could assume a spiral conformation found in active hexameric/dodecameric AAA+ chaperone structures. Vertical positioning of the middle domain accounts for the head-to-head arrangement of two heptameric rings. Biochemical activity assays with site-directed mutagenesis confirmed the critical roles of residues both in the integrity of the tetradecameric arrangement and activities of ClpL. Non-conserved Q321 and R670 are crucial in the heptameric ring assembly of ClpL. These results establish that ClpL is a functionally active tetradecamer, clearly distinct from hexameric/dodecameric AAA+ chaperones
URI
https://pr.ibs.re.kr/handle/8788114/7569
DOI
10.1096/fj.202000843R
ISSN
0892-6638
Appears in Collections:
Pioneer Research Center for Biomolecular and Cellular Structure(바이오분자 및 세포구조 연구단) > Protein Communication Group(단백질 커뮤니케이션 그룹) > 1. Journal Papers (저널논문)
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