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Hong, Seon Pyo
혈관 연구단
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Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2

DC Field Value Language
dc.contributor.authorJeonghwan Youk-
dc.contributor.authorTaewoo Kim-
dc.contributor.authorKelly V. Evans-
dc.contributor.authorYoung-Il Jeong-
dc.contributor.authorYongsuk Hur-
dc.contributor.authorSeon Pyo Hong-
dc.contributor.authorJe Hyoung Kim-
dc.contributor.authorKijong Yi,-
dc.contributor.authorSu Yeon Kim-
dc.contributor.authorKwon Joong Na-
dc.contributor.authorThomas Bleazard-
dc.contributor.authorHo Min Kim-
dc.contributor.authorMick Fellows-
dc.contributor.authorKrishnaa T. Mahbubani-
dc.contributor.authorKourosh Saeb-Parsy-
dc.contributor.authorSeon Young Kim-
dc.contributor.authorYoung Tae Kim-
dc.contributor.authorGou Young Koh-
dc.contributor.authorByeong-Sun Choi-
dc.contributor.authorYoung Seok Ju-
dc.contributor.authorJoo-Hyeon Lee-
dc.date.accessioned2020-12-22T02:21:49Z-
dc.date.accessioned2020-12-22T02:21:49Z-
dc.date.available2020-12-22T02:21:49Z-
dc.date.available2020-12-22T02:21:49Z-
dc.date.created2020-12-03-
dc.date.issued2020-12-03-
dc.identifier.issn1934-5909-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/7513-
dc.description.abstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of a present pandemic, infects human lung alveolar type 2 (hAT2) cells. Characterizing pathogenesis is crucial for developing vaccines and therapeutics. However. the lack of models mirroring the cellular physiology and pathology of hAT2 cells limits the study. Here, we develop a feeder-free, long-term, three-dimensional (3D) culture technique for hAT2 cells derived from primary human lung tissue and investigate infection response to SARS-CoV-2. By imaging-based analysis and single-cell transcriptome profiling, we reveal rapid viral replication and the increased expression of interferon-associated genes and proinflammatory genes in infected hAT2 cells, indicating a robust endogenous innate immune response. Further tracing of viral mutations acquired during transmission identifies full infection of individual cells effectively from a single viral entry. Our study provides deep insights into the pathogenesis of SARS-CoV-2 and the application of defined 3D hAT2 cultures as models for respiratory diseases.-
dc.description.uri1-
dc.language영어-
dc.publisherCELL PRESS-
dc.subject3D cultures-
dc.subjectalveolar stem cells-
dc.subjectCOVID-19-
dc.subjectelectron microscopy-
dc.subjecthuman alveolar type 2 cells-
dc.subjectinfection-
dc.subjectinterferon-
dc.subjectinterferon-stimulating genes-
dc.subjectSARS-CoV-2-
dc.subjectsingle-cell RNA-seq-
dc.titleThree-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000596086200007-
dc.identifier.scopusid2-s2.0-85095831044-
dc.identifier.rimsid73842-
dc.contributor.affiliatedAuthorSeon Pyo Hong-
dc.contributor.affiliatedAuthorHo Min Kim-
dc.contributor.affiliatedAuthorGou Young Koh-
dc.identifier.doi10.1016/j.stem.2020.10.004-
dc.identifier.bibliographicCitationCELL STEM CELL, v.27, no.6, pp.905 - +-
dc.citation.titleCELL STEM CELL-
dc.citation.volume27-
dc.citation.number6-
dc.citation.startPage905-
dc.citation.endPage+-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaCell Biology-
dc.relation.journalWebOfScienceCategoryCell & Tissue Engineering-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.subject.keywordAuthor3D cultures-
dc.subject.keywordAuthoralveolar stem cells-
dc.subject.keywordAuthorCOVID-19-
dc.subject.keywordAuthorelectron microscopy-
dc.subject.keywordAuthorhuman alveolar type 2 cells-
dc.subject.keywordAuthorinfection-
dc.subject.keywordAuthorinterferon-
dc.subject.keywordAuthorinterferon-stimulating genes-
dc.subject.keywordAuthorSARS-CoV-2-
dc.subject.keywordAuthorsingle-cell RNA-seq-
Appears in Collections:
Center for Vascular Research(혈관 연구단) > 1. Journal Papers (저널논문)
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