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HS-146, a novel phosphoinositide 3-kinase alpha inhibitor, induces the apoptosis and inhibits the metastatic ability of human breast cancer cells

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Title
HS-146, a novel phosphoinositide 3-kinase alpha inhibitor, induces the apoptosis and inhibits the metastatic ability of human breast cancer cells
Author(s)
Ok Hyeon Kim; Ju‑Hee Lee; Shinmee Mah; Sung Yun Park; Sungwoo Hong; Soon‑Sun Hong
Publication Date
2020-06
Journal
INTERNATIONAL JOURNAL OF ONCOLOGY, v.56, no.6, pp.1509 - 1520
Publisher
SPANDIDOS PUBL LTD
Abstract
The phosphoinositide 3-kinase (PI3K) signaling pathway plays an important role in human cancer as it regulates critical cellular functions, such as survival, proliferation and metabolism. In the present study, a novel PI3K alpha inhibitor (HS-146) was synthesized and its anticancer effects on MCF-7, MDA-MB-231, SKBR3 and BT-474 human breast cancer cell lines were confirmed. HS-146 was found to be most effective in inhibiting the proliferation of MCF-7 cells and in inducing cell cycle arrest in the G0/G1 phase by downregulating cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)2 and Cdk4, and upregulating p21(Waf1/Cip1) protein levels in this cell line. The induction of apoptosis by HS-146 was confirmed by DAPI staining and western blot analysis. Cell shrinkage and nuclear condensation, which are typical morphological markers of apoptosis, were increased by HS-146 in the MCF-7 cells in a concentration-dependent manner, and HS-146 also increased the protein expression levels of cleaved poly(ADP-ribose) polymerase (PARP) and decreased the protein expression levels of Mcl-1 and caspase-7. In addition, HS-146 effectively decreased the phosphorylation levels of downstream PI3K effectors, such as Akt, mammalian target of rapamycin (mTOR), glycogen synthase kinase 3 beta (GSK3 beta), p70S6K1 and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1). Hypoxia-inducible factor (HIF)-1 alpha and vascular endothelial growth factor (VEGF) expression were also suppressed by HS-146 under hypoxic conditions, and HS-146 inhibited the migration and invasion of MCF-7 cells in a concentration-dependent manner. On the whole, the findings of the present study suggest that HS-146, a novel PI3K alpha inhibitor, may be an effective novel therapeutic candidate that suppresses breast cancer proliferation and metastasis by inhibiting the PI3K/Akt/mTOR pathway
URI
https://pr.ibs.re.kr/handle/8788114/7122
ISSN
1019-6439
Appears in Collections:
Center for Catalytic Hydrocarbon Functionalizations(분자활성 촉매반응 연구단) > Journal Papers (저널논문)
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