Interpretation of EBV infection in pan-cancer genome considering viral life cycle: LiEB (Life cycle of Epstein-Barr virus)
DC Field | Value | Language |
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dc.contributor.author | Song H. | - |
dc.contributor.author | Lim Y. | - |
dc.contributor.author | Im H. | - |
dc.contributor.author | Bae J.M. | - |
dc.contributor.author | Kang G.H. | - |
dc.contributor.author | Junhak Ahn | - |
dc.contributor.author | Daehyun Baek | - |
dc.contributor.author | Kim T.-Y. | - |
dc.contributor.author | Yoon S.-S. | - |
dc.contributor.author | Koh Y. | - |
dc.date.available | 2019-08-19T02:06:43Z | - |
dc.date.created | 2019-03-19 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/6002 | - |
dc.description.abstract | We report a novel transcriptomic analysis workflow called LiEB (Life cycle of Epstein-Barr virus) to characterize distributions of oncogenic virus, Epstein-Barr virus (EBV) infection in human tumors. We analyzed 851 The Cancer Genome Atlas whole-transcriptome sequencing (WTS) data to investigate EBV infection by life cycle information using three-step LiEB workflow: 1) characterize virus infection generally; 2) align transcriptome sequences against a hybrid human-EBV genome, and 3) quantify EBV gene expression. Our results agreed with EBV infection status of public cell line data. Analysis in stomach adenocarcinoma identified EBV-positive cases involving PIK3CA mutations and/or CDKN2A silencing with biologically more determination, compared to previous reports. In this study, we found that a small number of colorectal adenocarcinoma cases involved with EBV lytic gene expression. Expression of EBV lytic genes was also observed in 3% of external colon cancer cohort upon WTS analysis. Gene set enrichment analysis showed elevated expression of genes related to E2F targeting and interferon-gamma responses in EBV-associated tumors. Finally, we suggest that interpretation of EBV life cycle is essential when analyzing its infection in tumors, and LiEB provides high capability of detecting EBV-positive tumors. Observation of EBV lytic gene expression in a subset of colon cancers warrants further research. © 2019, The Author(s) | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Interpretation of EBV infection in pan-cancer genome considering viral life cycle: LiEB (Life cycle of Epstein-Barr virus) | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000460381600038 | - |
dc.identifier.scopusid | 2-s2.0-85062587217 | - |
dc.identifier.rimsid | 67533 | - |
dc.contributor.affiliatedAuthor | Junhak Ahn | - |
dc.contributor.affiliatedAuthor | Daehyun Baek | - |
dc.identifier.doi | 10.1038/s41598-019-39706-0 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.9, no.1, pp.3465 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 3465 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |