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유전체교정연구단
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Web-based design and analysis tools for CRISPR base editing

DC Field Value Language
dc.contributor.authorHwang G.-H.-
dc.contributor.authorPark J.-
dc.contributor.authorKayeong Lim-
dc.contributor.authorKim S.-
dc.contributor.authorYu J.-
dc.contributor.authorYu E.-
dc.contributor.authorSang-Tae Kim-
dc.contributor.authorEils R.-
dc.contributor.authorJin-Soo Kim-
dc.contributor.authorBae S.-
dc.date.available2019-05-02T08:10:21Z-
dc.date.created2019-01-15-
dc.date.issued2018-12-
dc.identifier.issn1471-2105-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/5770-
dc.description.abstractBackground: As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing tools will be very useful for gene correction because they can produce highly specific DNA substitutions without the introduction of any donor DNA, but dedicated web-based tools to facilitate the use of such tools have not yet been developed. Results: We present two web tools for base editors, named BE-Designer and BE-Analyzer. BE-Designer provides all possible base editor target sequences in a given input DNA sequence with useful information including potential off-target sites. BE-Analyzer, a tool for assessing base editing outcomes from next generation sequencing (NGS) data, provides information about mutations in a table and interactive graphs. Furthermore, because the tool runs client-side, large amounts of targeted deep sequencing data (< 1 GB) do not need to be uploaded to a server, substantially reducing running time and increasing data security. BE-Designer and BE-Analyzer can be freely accessed at http://www.rgenome.net/be-designer/ and http://www.rgenome.net/be-analyzer /, respectively. Conclusion: We develop two useful web tools to design target sequence (BE-Designer) and to analyze NGS data from experimental results (BE-Analyzer) for CRISPR base editors. ? 2018 The Author(s)-
dc.description.uri1-
dc.language영어-
dc.publisherBIOMED CENTRAL LTD-
dc.subjectBase editing-
dc.subjectCRISPR-
dc.subjectGenome editing-
dc.subjectNGS analysis-
dc.subjectWeb-based tool-
dc.titleWeb-based design and analysis tools for CRISPR base editing-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000454396500002-
dc.identifier.scopusid2-s2.0-85059242230-
dc.identifier.rimsid66696-
dc.contributor.affiliatedAuthorKayeong Lim-
dc.contributor.affiliatedAuthorSang-Tae Kim-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1186/s12859-018-2585-4-
dc.identifier.bibliographicCitationBMC BIOINFORMATICS, v.19, no.1, pp.542-
dc.citation.titleBMC BIOINFORMATICS-
dc.citation.volume19-
dc.citation.number1-
dc.citation.startPage542-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordAuthorBase editing-
dc.subject.keywordAuthorCRISPR-
dc.subject.keywordAuthorGenome editing-
dc.subject.keywordAuthorNGS analysis-
dc.subject.keywordAuthorWeb-based tool-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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성과추가_1812_사사X_김상태_BMC Bioinformatics.pdfDownload

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