Structural and functional study of ChuY from Escherichia coli strain CFT073

Cited 1 time in webofscience Cited 0 time in scopus
116 Viewed 19 Downloaded
Title
Structural and functional study of ChuY from Escherichia coli strain CFT073
Author(s)
Hun Kim; Akhilesg Kumar Chaurasia; Truc Kim; Jongkeun Choi; Sung Chul Ha; Doyoun Kim; Kyeong Kyu Kim
Publication Date
2017-01
Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.482, no.4, pp.1176 - 1182
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
The uropathogenic Escherichia coli strain CFT073 contains multiple iron and heme transport systems, which facilitate infection of the host urinary tract. To elucidate the molecular and cellular function of ChuY, a hypothetical gene in the heme degradation/utilization pathway, we solved the crystal structure of ChuY at 2.4 angstrom resolution. ChuY has high structural homology with human biliverdin and flavin reductase. We confirmed that ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. A chuY deletion-insertion strain showed reduced survival potential compared to wild-type and complemented strains in mammalian cells. Current results suggest ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of E. coli CFT073. (C) 2016 Elsevier Inc. All rights reserved
URI
https://pr.ibs.re.kr/handle/8788114/4822
ISSN
0006-291X
Appears in Collections:
Center for Synaptic Brain Dysfunctions(시냅스 뇌질환 연구단) > Journal Papers (저널논문)
Files in This Item:
김도연_Structural.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse