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식물노화·수명연구단
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The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain

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dc.contributor.authorMooseok Kang-
dc.contributor.authorSangyeol Kim-
dc.contributor.authorHyo Jung Kim-
dc.contributor.authorShrestha, P-
dc.contributor.authorYun, JH-
dc.contributor.authorBong-Kwan Phee-
dc.contributor.authorWeontae Lee-
dc.contributor.authorHong Gil Nam-
dc.contributor.authorIksoo Chang-
dc.date.available2018-07-18T02:06:59Z-
dc.date.created2018-04-16ko
dc.date.issued2018-01-
dc.identifier.issn2211-1247-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/4707-
dc.description.abstractThe affinity of transcription factors (TFs) for their target DNA is a critical determinant of gene expression. Whether the DNA-binding domain (DBD) of TFs alone can regulate binding affinity to DNA is an important question for identifying the design principle of TFs. We studied ANAC019, a member of the NAC TF family of proteins in Arabidopsis, and found a well-conserved histidine switch located in its DBD, which regulates both homodimerization and transcriptional control of the TF through H135 protonation. We found that the removal of a C-terminal intrinsically disordered region (IDR) in the TF abolished the pH-dependent binding of the N-terminal DBD to DNA. We propose a mechanism in which long-range electrostatic interactions between DNA and the negatively charged C-terminal IDR turns on the pH dependency of the DNA-binding affinity of the N-terminal DBD (c) 2018 The Authors-
dc.description.uri1-
dc.language영어-
dc.publisherCELL PRESS-
dc.subjectelectric dipole moment-
dc.subjecthistidine switch-
dc.subjectintrinsically disordered region-
dc.subjectpH-tuned DNA-binding affinity-
dc.subjecttranscription factor-
dc.titleThe C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000423898500005-
dc.identifier.scopusid2-s2.0-85044864664-
dc.identifier.rimsid62984ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorHyo Jung Kim-
dc.contributor.affiliatedAuthorBong-Kwan Phee-
dc.contributor.affiliatedAuthorHong Gil Nam-
dc.identifier.doi10.1016/j.celrep.2018.01.002-
dc.identifier.bibliographicCitationCELL REPORTS, v.22, no.5, pp.1141 - 1150-
dc.citation.titleCELL REPORTS-
dc.citation.volume22-
dc.citation.number5-
dc.citation.startPage1141-
dc.citation.endPage1150-
dc.date.scptcdate2018-10-01-
dc.description.scptc0-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusARABIDOPSIS-THALIANA-
dc.subject.keywordPlusSIGNAL-TRANSDUCTION-
dc.subject.keywordPlusFACTOR FAMILY-
dc.subject.keywordPlusMEMBER-
dc.subject.keywordAuthorelectric dipole moment-
dc.subject.keywordAuthorhistidine switch-
dc.subject.keywordAuthorintrinsically disordered region-
dc.subject.keywordAuthorpH-tuned DNA-binding affinity-
dc.subject.keywordAuthortranscription factor-
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
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Cell Reports_Vol. 22(5) pp. 1141-1150.pdfDownload

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