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식물노화·수명연구단
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Transcriptome analysis of wild-type and afsS deletion mutant strains identifies synergistic transcriptional regulator of afsS for a high antibiotic-producing strain of Streptomyces coelicolor A3(2)

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dc.contributor.authorMin Woo Kim-
dc.contributor.authorBo-Rahm Lee-
dc.contributor.authorSungYong You-
dc.contributor.authorEun-Jung Kim-
dc.contributor.authorJi-Nu Kim-
dc.contributor.authorEunjung Song-
dc.contributor.authorYung-Hun Yang-
dc.contributor.authorDaehee Hwang-
dc.contributor.authorByung-Gee Kim-
dc.date.available2018-04-27T06:31:10Z-
dc.date.created2018-02-28-
dc.date.issued2018-04-
dc.identifier.issn0175-7598-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/4434-
dc.description.abstractMost secondary metabolism in Actinobacteria is controlled by multi-layered, gene-regulatory networks. These regulatory mechanisms are not easily identified due to their complexity. As a result, when a strong transcriptional regulator (TR) governs activation of biosynthetic pathways of target antibiotics such as actinorhodin (ACT), additional enhancement of the biosynthesis is difficult in combination with other TRs. To find out any Bsynergistic transcriptional regulators (sTRs)^ that show an additive effect on the major, often strong, transcriptional regulator (mTR), here, we performed a clustering analysis using the transcriptome datasets of an mTR deletion mutant and wild-type strain. In the case of ACT biosynthesis in Streptomyces coelicolor, PhoU (SCO4228) and RsfA (SCO4677) were selected through the clustering analysis, using AfsS (SCO4425) as a model mTR, and experimentally validated their roles as sTRs. Furthermore, through analysis of synergistic effects, we were able to suggest a novel regulation mechanism and formulate a strategy to maximize the synergistic effect. In the case of the double TR mutant strain (ΔrsfA pIBR25::afsS), it was confirmed that the increase of cell mass was the major cause of the synergistic effect. Therefore, the strategy to increase the cell mass of double mutant was further attempted by optimizing the expression of efflux pump, which resulted in 2-fold increase in the cell mass and 24-fold increase in the production of ACT. This result is the highest ACT yield from S. coelicolor ever reported. (c) Springer-Verlag GmbH Germany, part of Springer Nature 2018-
dc.description.uri1-
dc.language영어-
dc.publisherSPRINGER-
dc.subjectCombination of transcriptional regulators . Clustering analysis . Time-series transcriptome . Actinorhodin .-
dc.subjectStreptomyces coelicolor-
dc.titleTranscriptome analysis of wild-type and afsS deletion mutant strains identifies synergistic transcriptional regulator of afsS for a high antibiotic-producing strain of Streptomyces coelicolor A3(2)-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000427579600026-
dc.identifier.scopusid2-s2.0-85042092519-
dc.identifier.rimsid62738ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorDaehee Hwang-
dc.identifier.doi10.1007/s00253-018-8838-3-
dc.identifier.bibliographicCitationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.102, no.7, pp.3242 - 3253-
dc.citation.titleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY-
dc.citation.volume102-
dc.citation.number7-
dc.citation.startPage3242-
dc.citation.endPage3253-
dc.date.scptcdate2018-10-01-
dc.description.scptc0-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusSECONDARY METABOLITE BIOSYNTHESIS-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusCLUSTER-
dc.subject.keywordPlusDISCOVERY-
dc.subject.keywordPlusLIVIDANS-
dc.subject.keywordPlusADPA-
dc.subject.keywordPlusPHOP-
dc.subject.keywordAuthorCombination of transcriptional regulators-
dc.subject.keywordAuthorClustering analysis-
dc.subject.keywordAuthorTime-series transcriptome-
dc.subject.keywordAuthorActinorhodin-
dc.subject.keywordAuthorStreptomyces coelicolor-
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
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