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유전체교정연구단
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Rescue of high-specificity Cas9 variants using sgRNAs with matched 5 ' nucleotides

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dc.contributor.authorSojung Kim-
dc.contributor.authorTaegeun Bae-
dc.contributor.authorJaewoong Hwang-
dc.contributor.authorJin-Soo Kim-
dc.date.available2018-01-02T00:27:47Z-
dc.date.created2017-12-12-
dc.date.issued2017-11-
dc.identifier.issn1474-760X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/4120-
dc.description.abstractWe report that engineered Cas9 variants with improved specificity-eCas9-1.1 and Cas9-HF1-are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5' terminus, relative to target DNA sequences. Because the nucleotide at the 5' end of sgRNAs, expressed under the control of the commonly-used U6 promoter, is fixed to a guanine, these attenuated Cas9 variants are not useful at many target sites. By using sgRNAs with matched 5' nucleotides, produced by linking them to a self-cleaving ribozyme, the editing activity of Cas9 variants can be rescued without sacrificing high specificity. © The Author(s). 2017-
dc.description.uri1-
dc.language영어-
dc.publisherBIOMED CENTRAL LTD-
dc.subjectCRISPR-Cas-
dc.subjectOff-target effect-
dc.subjectEngineered Cas9 variants-
dc.subjectHammerhead ribozyme-linked sgRNA-
dc.titleRescue of high-specificity Cas9 variants using sgRNAs with matched 5 ' nucleotides-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000415306700002-
dc.identifier.scopusid2-s2.0-85034565473-
dc.identifier.rimsid61481ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorSojung Kim-
dc.contributor.affiliatedAuthorTaegeun Bae-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1186/s13059-017-1355-3-
dc.identifier.bibliographicCitationGENOME BIOLOGY, v.18, no.1, pp.218-
dc.citation.titleGENOME BIOLOGY-
dc.citation.volume18-
dc.citation.number1-
dc.citation.startPage218-
dc.date.scptcdate2018-10-01-
dc.description.wostc5-
dc.description.scptc3-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusHUMAN-CELLS-
dc.subject.keywordPlusGUIDE RNAS-
dc.subject.keywordPlusGENOME-
dc.subject.keywordPlusNUCLEASES-
dc.subject.keywordPlusENDONUCLEASE-
dc.subject.keywordAuthorCRISPR-Cas-
dc.subject.keywordAuthorOff-target effect-
dc.subject.keywordAuthorEngineered Cas9 variants-
dc.subject.keywordAuthorHammerhead ribozyme-linked sgRNA-
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Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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