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Genome-wide Mapping of DROSHA Cleavage Sites on Primary MicroRNAs and Noncanonical Substrates

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dc.contributor.authorBaekgyu Kim-
dc.contributor.authorKyowon Jeong-
dc.contributor.authorV. Narry Kim-
dc.date.available2017-09-05T05:25:17Z-
dc.date.created2017-06-19-
dc.date.issued2017-04-
dc.identifier.issn1097-2765-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/3744-
dc.description.abstractMicroRNA (miRNA) maturation is initiated by DROSHA, a double-stranded RNA (dsRNA)-specific RNase III enzyme. By cleaving primary miRNAs (pri-miRNAs) at specific positions, DROSHA serves as a main determinant of miRNA sequences and a highly selective gatekeeper for the canonical miRNA pathway. However, the sites of DROSHA-mediated processing have not been annotated, and it remains unclear to what extent DROSHA functions outside the miRNA pathway. Here, we establish a protocol termed “formaldehyde crosslinking, immunoprecipitation, and sequencing (fCLIP-seq),” which allows identification of DROSHA cleavage sites at single-nucleotide resolution. fCLIP identifies numerous processing sites, suggesting widespread end modifications during miRNA maturation. fCLIP also finds many pri-miRNAs that undergo alternative processing, yielding multiple miRNA isoforms. Moreover, we discovered dozens of DROSHA substrates on non-miRNA loci, which may serve as cis-elements for DROSHA-mediated gene regulation. We anticipate that fCLIP-seq could be a general tool for investigating interactions between dsRNA-binding proteins and structured RNAs. © 2017 Elsevier Inc-
dc.languageENG-
dc.publisherCELL PRESS-
dc.titleGenome-wide Mapping of DROSHA Cleavage Sites on Primary MicroRNAs and Noncanonical Substrates-
dc.typeArticle-
dc.type.rimsA-
dc.identifier.wosid000399553100011-
dc.identifier.scopusid2-s2.0-85018628542-
dc.description.wostc9-
dc.date.tcdate2018-10-01-
dc.date.scptcdate2018-10-01-
dc.subject.keywordCLIP-seq-
dc.subject.keywordDGCR8-
dc.subject.keywordDROSHA-
dc.subject.keywordformaldehyde crosslinking-
dc.subject.keywordmicroprocessor-
dc.subject.keywordmicroRNA-
dc.subject.keywordpri-miRNA-
dc.subject.keywordRNA-
dc.subject.keywordsequencing-
dc.contributor.affiliatedAuthorBaekgyu Kim-
dc.contributor.affiliatedAuthorV. Narry Kim-
dc.identifier.bibliographicCitationMOLECULAR CELL, v.66, no.2, pp.258 - 269-
dc.description.scptc9-
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Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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