Genome-wide Mapping of DROSHA Cleavage Sites on Primary MicroRNAs and Noncanonical Substrates
DC Field | Value | Language |
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dc.contributor.author | Baekgyu Kim | - |
dc.contributor.author | Kyowon Jeong | - |
dc.contributor.author | V. Narry Kim | - |
dc.date.available | 2017-09-05T05:25:17Z | - |
dc.date.created | 2017-06-19 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/3744 | - |
dc.description.abstract | MicroRNA (miRNA) maturation is initiated by DROSHA, a double-stranded RNA (dsRNA)-specific RNase III enzyme. By cleaving primary miRNAs (pri-miRNAs) at specific positions, DROSHA serves as a main determinant of miRNA sequences and a highly selective gatekeeper for the canonical miRNA pathway. However, the sites of DROSHA-mediated processing have not been annotated, and it remains unclear to what extent DROSHA functions outside the miRNA pathway. Here, we establish a protocol termed “formaldehyde crosslinking, immunoprecipitation, and sequencing (fCLIP-seq),” which allows identification of DROSHA cleavage sites at single-nucleotide resolution. fCLIP identifies numerous processing sites, suggesting widespread end modifications during miRNA maturation. fCLIP also finds many pri-miRNAs that undergo alternative processing, yielding multiple miRNA isoforms. Moreover, we discovered dozens of DROSHA substrates on non-miRNA loci, which may serve as cis-elements for DROSHA-mediated gene regulation. We anticipate that fCLIP-seq could be a general tool for investigating interactions between dsRNA-binding proteins and structured RNAs. © 2017 Elsevier Inc | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.subject | CLIP-seq | - |
dc.subject | DGCR8 | - |
dc.subject | DROSHA | - |
dc.subject | formaldehyde crosslinking | - |
dc.subject | microprocessor | - |
dc.subject | microRNA | - |
dc.subject | pri-miRNA | - |
dc.subject | RNA | - |
dc.subject | sequencing | - |
dc.title | Genome-wide Mapping of DROSHA Cleavage Sites on Primary MicroRNAs and Noncanonical Substrates | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000399553100011 | - |
dc.identifier.scopusid | 2-s2.0-85018628542 | - |
dc.identifier.rimsid | 59681 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Baekgyu Kim | - |
dc.contributor.affiliatedAuthor | Kyowon Jeong | - |
dc.contributor.affiliatedAuthor | V. Narry Kim | - |
dc.identifier.doi | 10.1016/j.molcel.2017.03.013 | - |
dc.identifier.bibliographicCitation | MOLECULAR CELL, v.66, no.2, pp.258 - 269 | - |
dc.citation.title | MOLECULAR CELL | - |
dc.citation.volume | 66 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 258 | - |
dc.citation.endPage | 269 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 9 | - |
dc.description.scptc | 9 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | CLIP-seq | - |
dc.subject.keywordAuthor | DGCR8 | - |
dc.subject.keywordAuthor | DROSHA | - |
dc.subject.keywordAuthor | formaldehyde crosslinking | - |
dc.subject.keywordAuthor | microprocessor | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | pri-miRNA | - |
dc.subject.keywordAuthor | RNA | - |
dc.subject.keywordAuthor | sequencing | - |