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유전체교정연구단
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Highly efficient RNA-guided base editing in mouse embryosHighly Cited Paper

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dc.contributor.authorKyoungmi Kim-
dc.contributor.authorSeuk-Min Ryu-
dc.contributor.authorSang-Tae Kim-
dc.contributor.authorGayoung Baek-
dc.contributor.authorDaesik Kim-
dc.contributor.authorKayeong Lim-
dc.contributor.authorEugene Chung-
dc.contributor.authorSunghyun Kim-
dc.contributor.authorJin-Soo Kim-
dc.date.available2017-09-05T05:16:07Z-
dc.date.created2017-06-19-
dc.date.issued2017-05-
dc.identifier.issn1087-0156-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/3715-
dc.description.abstractBase editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectID HUMAN-CELLS-
dc.subjectCAS9-
dc.subjectENDONUCLEASES-
dc.subjectMICE-
dc.subjectSPECIFICITY-
dc.subjectMUTAGENESIS-
dc.subjectNICKASES-
dc.subjectKNOCKOUT-
dc.titleHighly efficient RNA-guided base editing in mouse embryos-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000400809800016-
dc.identifier.scopusid2-s2.0-85018918215-
dc.identifier.rimsid59672ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorKyoungmi Kim-
dc.contributor.affiliatedAuthorSeuk-Min Ryu-
dc.contributor.affiliatedAuthorSang-Tae Kim-
dc.contributor.affiliatedAuthorGayoung Baek-
dc.contributor.affiliatedAuthorKayeong Lim-
dc.contributor.affiliatedAuthorEugene Chung-
dc.contributor.affiliatedAuthorSunghyun Kim-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1038/nbt.3816-
dc.identifier.bibliographicCitationNATURE BIOTECHNOLOGY, v.35, no.5, pp.435 - 437-
dc.citation.titleNATURE BIOTECHNOLOGY-
dc.citation.volume35-
dc.citation.number5-
dc.citation.startPage435-
dc.citation.endPage437-
dc.date.scptcdate2018-10-01-
dc.description.wostc55-
dc.description.scptc59-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
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Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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