Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome
DC Field | Value | Language |
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dc.contributor.author | Kyle J. Hewitt | - |
dc.contributor.author | Duk Hyoung Kim | - |
dc.contributor.author | Prithvia Devadas | - |
dc.contributor.author | Rajalekshmi Prathibha | - |
dc.contributor.author | Chandler Zuo | - |
dc.contributor.author | Rajendran Sanalkumar | - |
dc.contributor.author | Kirby D Johnson | - |
dc.contributor.author | Yoon-A Kang | - |
dc.contributor.author | Jin Soo Kim | - |
dc.contributor.author | Colin N. Dewey | - |
dc.contributor.author | Sunduz Keles | - |
dc.contributor.author | Emery H. Bresnick | - |
dc.date.available | 2016-01-25T00:13:23Z | - |
dc.date.created | 2016-01-12 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/2342 | - |
dc.description.abstract | Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required for hematopoietic stem cell genesis in mice is mutated in a human immunodeficiency syndrome. Because +9.5 is the only cis-element known to mediate stem cell genesis, we devised a strategy to identify functionally comparable enhancers (‘‘+9.5-like’’) genome-wide. Gene editing revealed +9.5-like activity to mediate GATA-2 occupancy, chromatin opening, and transcriptional activation. A +9.5-like element resided in Samd14, which encodes a protein of unknown function. Samd14 increased hematopoietic progenitor levels/activity and promoted signaling by a pathway vital for hematopoietic stem/progenitor cell regulation (stem cell factor/c-Kit), and c-Kit rescued Samd14 lossof- function phenotypes. Thus, the hematopoietic stem/progenitor cell cistrome revealed a mediator of a signaling pathway that has broad importance for stem/progenitor cell biology. (c) 2015 Elsevier Inc. | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.title | Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000360987300007 | - |
dc.identifier.scopusid | 2-s2.0-84937162292 | - |
dc.identifier.rimsid | 21975 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Duk Hyoung Kim | - |
dc.contributor.affiliatedAuthor | Jin Soo Kim | - |
dc.identifier.doi | 10.1016/j.molcel.2015.05.020 | - |
dc.identifier.bibliographicCitation | MOLECULAR CELL, v.59, no.1, pp.62 - 74 | - |
dc.relation.isPartOf | MOLECULAR CELL | - |
dc.citation.title | MOLECULAR CELL | - |
dc.citation.volume | 59 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 62 | - |
dc.citation.endPage | 74 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 16 | - |
dc.description.scptc | 16 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | GENOME-WIDE ANALYSIS | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR GATA-2 | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CHROMATIN OCCUPANCY | - |
dc.subject.keywordPlus | FACTOR COMPLEXES | - |
dc.subject.keywordPlus | DNA ELEMENTS | - |
dc.subject.keywordPlus | MOUSE GENOME | - |
dc.subject.keywordPlus | CIS-ELEMENT | - |
dc.subject.keywordPlus | GENE LOCUS | - |
dc.subject.keywordPlus | PROTEIN | - |