Functional and physical interaction of diacylglycerol kinase ζ with protein kinase Cα is required for cerebellar long-term depression

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Title
Functional and physical interaction of diacylglycerol kinase ζ with protein kinase Cα is required for cerebellar long-term depression
Author(s)
Dongwon Lee; Yukio Yamamoto; Eunjoon Kim; Keiko Tanaka-Yamamoto
Publication Date
2015-11
Journal
JOURNAL OF NEUROSCIENCE, v.35, no.46, pp.15453 - 15465
Publisher
SOC NEUROSCIENCE
Abstract
The balance between positive and negative regulators required for synaptic plasticity must be well organized at synapses. Protein kinase Cα (PKCα) is a major mediator that triggers long-term depression (LTD) at synapses between parallel fibers and Purkinje cells in the cerebellum. However, the precise mechanisms involved in PKCα regulation are not clearly understood. Here, we analyzed the role of diacylglycerol kinase ζ (DGK ζ), a kinase that physically interacts with PKCα as well as postsynaptic density protein 95 (PSD-95) family proteins and functionally suppresses PKCα by metabolizing diacylglycerol (DAG), in the regulation of cerebellar LTD. In Purkinje cells of DGKζ-deficient mice, LTD was impaired and PKCα was less localized in dendrites and synapses. This impaired LTD was rescued by virus-driven expression of wild-type DGKζ, but not by a kinase-dead mutant DGKζ or a mutant lacking the ability to localize at synapses, indicating that both the kinase activity and synaptic anchoring functions of DGKζ are necessary for LTD. In addition, experiments using another DGKζ mutant and immunoprecipitation analysis revealed an inverse regulatory mechanism, in which PKCα phosphorylates, inactivates, and then is released from DGK ζ, is required for LTD. These results indicate that DGK ζ is localized to synapses, through its interaction with PSD-95 family proteins, to promote synaptic localization of PKCα, but maintains PKCα in a minimally activated state by suppressing local DAG until its activation and release from DGKζ during LTD. Such local and reciprocal regulation of positive and negative regulators may contribute to the fine-tuning of synaptic signaling. © 2015 the authors
URI
https://pr.ibs.re.kr/handle/8788114/1843
ISSN
0270-6474
Appears in Collections:
Center for Synaptic Brain Dysfunctions(시냅스 뇌질환 연구단) > Journal Papers (저널논문)
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