Spherically-Clustered Porous Au-Ag Alloy Nanoparticle Prepared by Partial Inhibition of Galvanic Replacement and Its Application for Efficient Multimodal Therapy

Abstract

The polyvinylpyrrolidone (PVP)-coated spherically clustered porous Old silver alloy nanoparticle (PVP-SPAN) was prepared by low temperature Mediated, partially inhibited galvanic replacement reaction followed by silver etching process. The prepared porous nanostructures exhibited excellent photothermal conversion efficiency under irradiation of near-infrared light (NIR) and allowed a high payload of both doxorubicin, (Dox) and thiolated dye-labeled oligonucleotide, DNAzyme (FDz). Especially, PVP-SPAN provided 10 times higher loading capacity for oligonucleotide than conventional hollow nanoshells due to increased pore diameter and surface-to-volume ratio. We demonstrated highly efficient chemo-thermo-gene multitherapy based on codelivery of Dox and FDz with NIR-mediated photothermal therapeutic effect using a model system of hepatitis C virus infected human liver cells (Huh7 human hepatocarcinoma cell line containing:hepatitis C virus NS3 gene replicon) compared to conventional hollow nanoshells