BROWSE

Related Scientist

rna's photo.

rna
rna연구단
more info

ITEM VIEW & DOWNLOAD

Time-resolved profiling of RNA binding proteins throughout the mRNA life cycle

DC Field Value Language
dc.contributor.authorYeon Choi-
dc.contributor.authorBuyeon Um-
dc.contributor.authorYongwoo Na-
dc.contributor.authorJeesoo Kim-
dc.contributor.authorJong-Seo Kim-
dc.contributor.authorV. Narry Kim-
dc.date.accessioned2024-05-27T05:50:05Z-
dc.date.available2024-05-27T05:50:05Z-
dc.date.created2024-05-07-
dc.date.issued2024-05-
dc.identifier.issn1097-2765-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/15190-
dc.description.abstractmRNAs continually change their protein partners throughout their lifetimes, yet our understanding of mRNA-protein complex (mRNP) remodeling is limited by a lack of temporal data. Here, we present time-resolved mRNA interactome data by performing pulse metabolic labeling with photoactivatable ribonucleoside in human cells, UVA crosslinking, poly(A)+ RNA isolation, and mass spectrometry. This longitudinal approach allowed the quantification of over 700 RNA binding proteins (RBPs) across ten time points. Overall, the sequential order of mRNA binding aligns well with known functions, subcellular locations, and molecular interactions. However, we also observed RBPs with unexpected dynamics: the transcription-export (TREX) complex recruited posttranscriptionally after nuclear export factor 1 (NXF1) binding, challenging the current view of transcription-coupled mRNA export, and stress granule proteins prevalent in aged mRNPs, indicating roles in late stages of the mRNA life cycle. To systematically identify mRBPs with unknown functions, we employed machine learning to compare mRNA binding dynamics with Gene Ontology (GO) annotations. Our data can be explored at chronology.rna.snu.ac.kr. © 2024 Elsevier Inc.-
dc.language영어-
dc.publisherCell Press-
dc.titleTime-resolved profiling of RNA binding proteins throughout the mRNA life cycle-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid001238537900001-
dc.identifier.scopusid2-s2.0-85191331694-
dc.identifier.rimsid83021-
dc.contributor.affiliatedAuthorYeon Choi-
dc.contributor.affiliatedAuthorBuyeon Um-
dc.contributor.affiliatedAuthorYongwoo Na-
dc.contributor.affiliatedAuthorJeesoo Kim-
dc.contributor.affiliatedAuthorJong-Seo Kim-
dc.contributor.affiliatedAuthorV. Narry Kim-
dc.identifier.doi10.1016/j.molcel.2024.03.012-
dc.identifier.bibliographicCitationMolecular Cell, v.84, no.9, pp.1764 - 1782.e10-
dc.relation.isPartOfMolecular Cell-
dc.citation.titleMolecular Cell-
dc.citation.volume84-
dc.citation.number9-
dc.citation.startPage1764-
dc.citation.endPage1782.e10-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordAuthormass spectrometry-
dc.subject.keywordAuthormRNA binding dynamics-
dc.subject.keywordAuthormRNA life-cycle-
dc.subject.keywordAuthormRNA nuclear export-
dc.subject.keywordAuthorNXF1-
dc.subject.keywordAuthorpulse-chase labeling-
dc.subject.keywordAuthorRNA binding protein-
dc.subject.keywordAuthorRNA interactome capture-
dc.subject.keywordAuthorstress granule-
dc.subject.keywordAuthorTREX complex-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse