BROWSE

Related Scientist

rna's photo.

rna
rna연구단
more info

ITEM VIEW & DOWNLOAD

Structural atlas of human primary microRNAs generated by SHAPE-MaP

Cited 0 time in webofscience Cited 0 time in scopus
117 Viewed 0 Downloaded
Title
Structural atlas of human primary microRNAs generated by SHAPE-MaP
Author(s)
S. Chan Baek; Boseon Kim; Harim Jang; Kijun Kim; Il-Soo Park; Dal-Hee Min; V. Narry Kim
Publication Date
2024-03
Journal
Molecular Cell, v.84, no.6, pp.1158 - 1172
Publisher
Cell Press
Abstract
MicroRNA (miRNA) maturation is critically dependent on structural features of primary transcripts (pri-miRNAs). However, the scarcity of determined pri-miRNA structures has limited our understanding of miRNA maturation. Here, we employed selective 2′-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP), a high-throughput RNA structure probing method, to unravel the secondary structures of 476 high-confidence human pri-miRNAs. Our SHAPE-based structures diverge substantially from those inferred solely from computation, particularly in the apical loop and basal segments, underlining the need for experimental data in RNA structure prediction. By comparing the structures with high-throughput processing data, we determined the optimal structural features of pri-miRNAs. The sequence determinants are influenced substantially by their structural contexts. Moreover, we identified an element termed the bulged GWG motif (bGWG) with a 3′ bulge in the lower stem, which promotes processing. Our structure-function mapping better annotates the determinants of pri-miRNA processing and offers practical implications for designing small hairpin RNAs and predicting the impacts of miRNA mutations. © 2024 Elsevier Inc.
URI
https://pr.ibs.re.kr/handle/8788114/15094
DOI
10.1016/j.molcel.2024.02.005
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse