Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteinsHighly Cited Paper
DC Field | Value | Language |
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dc.contributor.author | Sojung Kim | - |
dc.contributor.author | Daesik Kim | - |
dc.contributor.author | Seung Woo Cho | - |
dc.contributor.author | Jungeun Kim | - |
dc.contributor.author | Jin-Soo Kim | - |
dc.date.available | 2015-04-21T09:14:10Z | - |
dc.date.created | 2014-08-11 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 1088-9051 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/1502 | - |
dc.description.abstract | RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas (CRISPR-associated) enable genome editing in human cell lines, animals, and plants, but are limited by off-target effects and unwanted integration of DNA segments derived from plasmids encoding Cas9 and guide RNA at both on-target and off-target sites in the genome. Here, we deliver purified recombinant Cas9 protein and guide RNA into cultured human cells including hard-to-transfect fibroblasts and pluripotent stem cells. RGEN ribonucleoproteins (RNPs) induce site-specific mutations at frequencies of up to 79%, while reducing off-target mutations associated with plasmid transfection at off-target sites that differ by one or two nucleotides from on-target sites. RGEN RNPs cleave chromosomal DNA almost immediately after delivery and are degraded rapidly in cells, reducing off-target effects. Furthermore, RNP delivery is less stressful to human embryonic stem cells, producing at least twofold more colonies than does plasmid transfection. | - |
dc.language | 영어 | - |
dc.publisher | COLD SPRING HARBOR LAB PRESS | - |
dc.title | Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000336662200012 | - |
dc.identifier.scopusid | 2-s2.0-84901834420 | - |
dc.identifier.rimsid | 340 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Sojung Kim | - |
dc.contributor.affiliatedAuthor | Daesik Kim | - |
dc.contributor.affiliatedAuthor | Seung Woo Cho | - |
dc.contributor.affiliatedAuthor | Jungeun Kim | - |
dc.contributor.affiliatedAuthor | Jin-Soo Kim | - |
dc.identifier.doi | 10.1101/gr.171322.113 | - |
dc.identifier.bibliographicCitation | GENOME RESEARCH, v.24, no.6, pp.1012 - 1019 | - |
dc.relation.isPartOf | GENOME RESEARCH | - |
dc.citation.title | GENOME RESEARCH | - |
dc.citation.volume | 24 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1012 | - |
dc.citation.endPage | 1019 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 375 | - |
dc.description.scptc | 394 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |