BROWSE

Related Scientist

suhyung,park's photo.

suhyung,park
유전체항상성연구단
more info

ITEM VIEW & DOWNLOAD

Short-range end resection requires ATAD5-mediated PCNA unloading for faithful homologous recombination

DC Field Value Language
dc.contributor.authorSu Hyung Park-
dc.contributor.authorNamwoo Kim-
dc.contributor.authorNalae Kang-
dc.contributor.authorEunjin Ryu-
dc.contributor.authorEun A. Lee-
dc.contributor.authorJae Sun Ra-
dc.contributor.authorAnton Gartner-
dc.contributor.authorSukhyun Kang-
dc.contributor.authorKyungjae Myung-
dc.contributor.authorKyoo-young Lee-
dc.date.accessioned2023-12-06T22:00:55Z-
dc.date.available2023-12-06T22:00:55Z-
dc.date.created2023-10-16-
dc.date.issued2023-10-
dc.identifier.issn0305-1048-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/14309-
dc.description.abstractHomologous recombination (HR) requires bidirectional end resection initiated by a nick formed close to a DNA double-strand break (DSB), dysregulation favoring error-prone DNA end-joining pathways. Here we investigate the role of the ATAD5, a PCNA unloading protein, in short-range end resection, long-range resection not being affected by ATAD5 deficiency. Rapid PCNA loading onto DNA at DSB sites depends on the RFC PCNA loader complex and MRE11-RAD50-NBS1 nuclease complexes bound to CtIP. Based on our cytological analyses and on an in vitro system for short-range end resection, we propose that PCNA unloading by ATAD5 is required for the completion of short-range resection. Hampering PCNA unloading also leads to failure to remove the KU70/80 complex from the termini of DSBs hindering DNA repair synthesis and the completion of HR. In line with this model, ATAD5-depleted cells are defective for HR, show increased sensitivity to camptothecin, a drug forming protein-DNA adducts, and an augmented dependency on end-joining pathways. Our study highlights the importance of PCNA regulation at DSB for proper end resection and HR. Graphical Abstract-
dc.language영어-
dc.publisherOXFORD UNIV PRESS-
dc.titleShort-range end resection requires ATAD5-mediated PCNA unloading for faithful homologous recombination-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid001068403600001-
dc.identifier.scopusid2-s2.0-85175357772-
dc.identifier.rimsid81940-
dc.contributor.affiliatedAuthorSu Hyung Park-
dc.contributor.affiliatedAuthorNamwoo Kim-
dc.contributor.affiliatedAuthorNalae Kang-
dc.contributor.affiliatedAuthorEunjin Ryu-
dc.contributor.affiliatedAuthorEun A. Lee-
dc.contributor.affiliatedAuthorJae Sun Ra-
dc.contributor.affiliatedAuthorAnton Gartner-
dc.contributor.affiliatedAuthorSukhyun Kang-
dc.contributor.affiliatedAuthorKyungjae Myung-
dc.contributor.affiliatedAuthorKyoo-young Lee-
dc.identifier.doi10.1093/nar/gkad776-
dc.identifier.bibliographicCitationNUCLEIC ACIDS RESEARCH, v.51, no.19, pp.10519 - 10535-
dc.relation.isPartOfNUCLEIC ACIDS RESEARCH-
dc.citation.titleNUCLEIC ACIDS RESEARCH-
dc.citation.volume51-
dc.citation.number19-
dc.citation.startPage10519-
dc.citation.endPage10535-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.subject.keywordPlusDOUBLE-STRAND-BREAK-
dc.subject.keywordPlusREPAIR PATHWAY CHOICE-
dc.subject.keywordPlusDNA-DAMAGE-
dc.subject.keywordPlusHUMAN ELG1-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusINHIBITORS-
dc.subject.keywordPlusMECHANISM-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusPHOSPHORYLATION-
dc.subject.keywordPlusIDENTIFICATION-
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse