Mono-Uridylation of Pre-MicroRNA as a Key Step in the Biogenesis of Group II let-7 MicroRNAs
DC Field | Value | Language |
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dc.contributor.author | Inha Heo | - |
dc.contributor.author | Minju Ha | - |
dc.contributor.author | Jaechul Lim | - |
dc.contributor.author | Mi-Jeong Yoon | - |
dc.contributor.author | Jong-Eun Park | - |
dc.contributor.author | S. Chul Kwon | - |
dc.contributor.author | Hyeshik Chang | - |
dc.contributor.author | V. Narry Kim | - |
dc.date.available | 2015-04-20T07:26:01Z | - |
dc.date.created | 2014-08-11 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/1424 | - |
dc.description.abstract | RNase III Drosha initiates microRNA (miRNA) maturation by cleaving a primary miRNA transcript and releasing a pre-miRNA with a 2 nt 3` overhang. Dicer recognizes the 2 nt 3` overhang structure to selectively process pre-miRNAs. Here, we find that, unlike prototypic pre-miRNAs #group I#, group II pre-miRNAs acquire a shorter #1 nt# 3` overhang from Drosha processing and therefore require a 3`-end mono-uridylation for Dicer processing. The majority of let-7 and miR-105 belong to group II. We identify TUT7/ZCCHC6, TUT4/ZCCHC11, and TUT2/PAPD4/GLD2 as the terminal uridylyl transferases responsible for pre-miRNA mono-uridylation. The TUTs act specifically on dsRNAs with a 1 nt 3` overhang, thereby creating a 2 nt 3` overhang. Depletion of TUTs reduces let-7 levels and disrupts let-7 function. Although the let-7 suppressor, Lin28, induces inhibitory oligo-uridylation in embryonic stem cells, mono-uridylation occurs in somatic cells lacking Lin28 to promote let-7 biogenesis. Our study reveals functional duality of uridylation and introduces TUT7/4/2 as components of the miRNA biogenesis pathway. | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.title | Mono-Uridylation of Pre-MicroRNA as a Key Step in the Biogenesis of Group II let-7 MicroRNAs | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000310529300010 | - |
dc.identifier.scopusid | 2-s2.0-84868153864 | - |
dc.identifier.rimsid | 116 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Inha Heo | - |
dc.contributor.affiliatedAuthor | Minju Ha | - |
dc.contributor.affiliatedAuthor | Jaechul Lim | - |
dc.contributor.affiliatedAuthor | Jong-Eun Park | - |
dc.contributor.affiliatedAuthor | S. Chul Kwon | - |
dc.contributor.affiliatedAuthor | Hyeshik Chang | - |
dc.contributor.affiliatedAuthor | V. Narry Kim | - |
dc.identifier.doi | 10.1016/j.cell.2012.09.022 | - |
dc.identifier.bibliographicCitation | CELL, v.151, no.3, pp.521 - 532 | - |
dc.relation.isPartOf | CELL | - |
dc.citation.title | CELL | - |
dc.citation.volume | 151 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 521 | - |
dc.citation.endPage | 532 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 128 | - |
dc.description.scptc | 129 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |