Functional viromic screens uncover regulatory RNA elements
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jenny J. Seo | - |
dc.contributor.author | Soo-Jin Jung | - |
dc.contributor.author | Jihye Yang | - |
dc.contributor.author | Da-Eun Choi | - |
dc.contributor.author | V. Narry Kim | - |
dc.date.accessioned | 2023-09-04T22:00:55Z | - |
dc.date.available | 2023-09-04T22:00:55Z | - |
dc.date.created | 2023-08-02 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/13873 | - |
dc.description.abstract | The number of sequenced viral genomes has surged recently, presenting an opportunity to understand viral diversity and uncover unknown regulatory mechanisms. Here, we conducted a screening of 30,367 viral segments from 143 species representing 96 genera and 37 families. Using a library of viral segments in 3′ UTR, we identified hundreds of elements impacting RNA abundance, translation, and nucleocytoplasmic distribution. To illustrate the power of this approach, we investigated K5, an element conserved in kobuviruses, and found its potent ability to enhance mRNA stability and translation in various contexts, including adeno-associated viral vectors and synthetic mRNAs. Moreover, we identified a previously uncharacterized protein, ZCCHC2, as a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. This study provides a unique resource for virus and RNA research and highlights the potential of the virosphere for biological discoveries. © 2023 Elsevier Inc. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Functional viromic screens uncover regulatory RNA elements | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 001047592300001 | - |
dc.identifier.scopusid | 2-s2.0-85165105428 | - |
dc.identifier.rimsid | 81377 | - |
dc.contributor.affiliatedAuthor | Jenny J. Seo | - |
dc.contributor.affiliatedAuthor | Soo-Jin Jung | - |
dc.contributor.affiliatedAuthor | Jihye Yang | - |
dc.contributor.affiliatedAuthor | Da-Eun Choi | - |
dc.contributor.affiliatedAuthor | V. Narry Kim | - |
dc.identifier.doi | 10.1016/j.cell.2023.06.007 | - |
dc.identifier.bibliographicCitation | Cell, v.186, no.15, pp.3291 - 3306.e21 | - |
dc.relation.isPartOf | Cell | - |
dc.citation.title | Cell | - |
dc.citation.volume | 186 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 3291 | - |
dc.citation.endPage | 3306.e21 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SAM DOMAIN | - |
dc.subject.keywordPlus | PEPTIDE IDENTIFICATION | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordAuthor | massively parallel reporter assay | - |
dc.subject.keywordAuthor | mixed tailing | - |
dc.subject.keywordAuthor | poly(A) tail | - |
dc.subject.keywordAuthor | post-transcriptional regulation | - |
dc.subject.keywordAuthor | RNA element | - |
dc.subject.keywordAuthor | TENT4 | - |
dc.subject.keywordAuthor | virus | - |
dc.subject.keywordAuthor | ZCCHC2 | - |
dc.subject.keywordAuthor | Aichi virus | - |
dc.subject.keywordAuthor | deadenylation | - |