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Characterization of passive microfluidic mixer with a three-dimensional zig-zag channel for cryo-EM sampling

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Title
Characterization of passive microfluidic mixer with a three-dimensional zig-zag channel for cryo-EM sampling
Author(s)
Lee, Byungjin; Kim, Minjoong; Oh, Seoyeon; Bi Lee, Dan; Seong-Gyu Lee; Ho Min Kim; Kim, Kyung Hyun; Song, Jihwan; Lee, Chang-Soo
Publication Date
2023-11
Journal
Chemical Engineering Science, v.281
Publisher
Elsevier Ltd
Abstract
Microfluidic mixing is an essential unit operation in miniaturized analytical systems. Achieving high mixing efficiency is considerably difficult in conventional microfluidic micromixers because the fluid flow is generally associated with a laminar flow regime and is dominated by molecular diffusion. In this study, different microfluidic passive mixer devices are designed, simulated, and tested. Passive microfluidic mixers, such as straight, 2D zig-zag, and 3D zig-zag geometries, are evaluated to assess the mixing efficiency of each layout. The mixing performance is investigated, and the comparison of experimental results with computational simulations determines the mixing efficiencies. We characterized the 3D zig-zag channel to perform a promising design for improving mixing in a microfluidic passive mixer device and can be optimized to enhance mixing efficiency by the appropriate length of the microfluidic mixer. A passive micromixer with a 3D zig-zag channel is further demonstrated by characterizing mixing at varying Reynolds numbers of 18.5 ≤ Re ≤ 55.5. Finally, we validate the utility of the mixing device for the screening of detergents for a preferential orientation issue of influenza A virus hemagglutinin protein particles in cryo-EM integrated with a microfluidic spraying approach. The results reported here are versatile, reproducible, and readily adaptable to various fields needing a fast and reliable aqueous phase mixing strategy.
URI
https://pr.ibs.re.kr/handle/8788114/13852
DOI
10.1016/j.ces.2023.119161
ISSN
0009-2509
Appears in Collections:
Pioneer Research Center for Biomolecular and Cellular Structure(바이오분자 및 세포구조 연구단) > Protein Communication Group(단백질 커뮤니케이션 그룹) > 1. Journal Papers (저널논문)
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