BROWSE

Related Scientist

RNA's photo.

RNA
RNA 연구단
more info

ITEM VIEW & DOWNLOAD

Binding of USP4 to cortactin enhances cell migration in HCT116 human colon cancer cells

Cited 0 time in webofscience Cited 0 time in scopus
179 Viewed 0 Downloaded
Title
Binding of USP4 to cortactin enhances cell migration in HCT116 human colon cancer cells
Author(s)
Yun, Sun-Il; Kwak, Chulhwan; Lee, Song-Yi; Shin, Sanghee; Oh, Changsuk; Jong-Seo Kim; Rhee, Hyun-Woo; Kim, Kyeong Kyu
Publication Date
2023-05
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, v.37, no.5, pp.e22900
Publisher
NLM (Medline)
Abstract
Ubiquitin-specific protease 4 (USP4) is highly overexpressed in colon cancer and acts as a potent protooncogenic protein by deubiquitinating β-catenin. However, its prominent roles in tumor formation and migration in cancer cells are not fully understood by its deubiquitinating enzyme (DUB) activity on β-catenin. Thus, we investigated an additional role of USP4 in cancer. In this study, we identified cortactin (CTTN), an actin-binding protein involved in the regulation of cytoskeleton dynamics and a potential prognostic marker for cancers, as a new cellular interacting partner of USP4 from proximal labeling of HCT116 cells. Additionally, the role of USP4 in CTTN activation and promotion of cell dynamics and migration was investigated in HCT116 cells. We confirmed that interacting of USP4 with CTTN increased cell movement. This finding was supported by the fact that USP4 overexpression in HCT116 cells with reduced expression of CTTN was insufficient to promote cell migration. Additionally, we observed that USP4 overexpression led to a significant increase in CTTN phosphorylation, which is a requisite mechanism for cell migration, by regulating Src/focal adhesion kinase (FAK) binding to CTTN and its activation. Our results suggest that USP4 plays a dual role in cancer progression, including stabilization of β-catenin as a DUB and interaction with CTTN to promote cell dynamics by inducing CTTN phosphorylation. Therefore, this study demonstrates that USP4 is important for cancer progression and is a good target for treating or preventing cancer. © 2023 Federation of American Societies for Experimental Biology.
URI
https://pr.ibs.re.kr/handle/8788114/13446
DOI
10.1096/fj.202201337RRR
ISSN
0892-6638
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse