Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelium
DC Field | Value | Language |
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dc.contributor.author | Young Woong Kim | - |
dc.contributor.author | Zara, Greta | - |
dc.contributor.author | Kang, HyunJun | - |
dc.contributor.author | Branciamore, Sergio | - |
dc.contributor.author | O’Meally, Denis | - |
dc.contributor.author | Feng, Yuxin | - |
dc.contributor.author | Kuan, Chia-Yi | - |
dc.contributor.author | Luo, Yingjun | - |
dc.contributor.author | Nelson, Michael S. | - |
dc.contributor.author | Brummer, Alex B. | - |
dc.contributor.author | Rockne, Russell | - |
dc.contributor.author | Chen, Zhen Bouman | - |
dc.contributor.author | Zheng, Yi | - |
dc.contributor.author | Cardoso, Angelo A. | - |
dc.contributor.author | Carlesso, Nadia | - |
dc.date.accessioned | 2023-01-26T02:30:35Z | - |
dc.date.available | 2023-01-26T02:30:35Z | - |
dc.date.created | 2022-12-06 | - |
dc.date.issued | 2022-11 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/12554 | - |
dc.description.abstract | © 2022, The Author(s).Heterogeneity of endothelial cell (EC) populations reflects their diverse functions in maintaining tissue’s homeostasis. However, their phenotypic, molecular, and functional properties are not entirely mapped. We use the Tie2-CreERT2;Rosa26-tdTomato reporter mouse to trace, profile, and cultivate primary ECs from different organs. As paradigm platform, we use this strategy to study bone marrow endothelial cells (BMECs). Single-cell mRNA sequencing of primary BMECs reveals that their diversity and native molecular signatures is transitorily preserved in an ex vivo culture that conserves key cell-to-cell microenvironment interactions. Macrophages sustain BMEC cellular diversity and expansion and preserve sinusoidal-like BMECs ex vivo. Endomucin expression discriminates BMECs in populations exhibiting mutually exclusive properties and distinct sinusoidal/arterial and tip/stalk signatures. In contrast to arterial-like, sinusoidal-like BMECs are short-lived, form 2D-networks, contribute to in vivo angiogenesis, and support hematopoietic stem/progenitor cells in vitro. This platform can be extended to other organs’ ECs to decode mechanistic information and explore therapeutics. | - |
dc.language | 영어 | - |
dc.publisher | Nature Research | - |
dc.title | Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelium | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000888905000009 | - |
dc.identifier.scopusid | 2-s2.0-85142467785 | - |
dc.identifier.rimsid | 79372 | - |
dc.contributor.affiliatedAuthor | Young Woong Kim | - |
dc.identifier.doi | 10.1038/s41467-022-34425-z | - |
dc.identifier.bibliographicCitation | Nature Communications, v.13, no.1 | - |
dc.relation.isPartOf | Nature Communications | - |
dc.citation.title | Nature Communications | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | HEMATOPOIETIC STEM | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | TIE2 ACTIVATION | - |
dc.subject.keywordPlus | TUBE FORMATION | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | NICHES | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | ALPHA | - |