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유전체교정연구단
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Targeted A-to-G base editing in human mitochondrial DNA with programmable deaminasesHighly Cited Paper

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dc.contributor.authorSung-Ik Cho-
dc.contributor.authorSeonghyun Lee-
dc.contributor.authorYoung Geun Mok-
dc.contributor.authorKayeong Lim-
dc.contributor.authorJaesuk Lee-
dc.contributor.authorJi Min Lee-
dc.contributor.authorEugene Chung-
dc.contributor.authorJin-Soo Kim-
dc.date.accessioned2022-08-26T22:03:28Z-
dc.date.available2022-08-26T22:03:28Z-
dc.date.created2022-06-07-
dc.date.issued2022-05-
dc.identifier.issn0092-8674-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/12260-
dc.description.abstract© 2022 The AuthorsMitochondrial DNA (mtDNA) editing paves the way for disease modeling of mitochondrial genetic disorders in cell lines and animals and also for the treatment of these diseases in the future. Bacterial cytidine deaminase DddA-derived cytosine base editors (DdCBEs) enabling mtDNA editing, however, are largely limited to C-to-T conversions in the 5′-TC context (e.g., TC-to-TT conversions), suitable for generating merely 1/8 of all possible transition (purine-to-purine and pyrimidine-to-pyrimidine) mutations. Here, we present transcription-activator-like effector (TALE)-linked deaminases (TALEDs), composed of custom-designed TALE DNA-binding arrays, a catalytically impaired, full-length DddA variant or split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein, which induce targeted A-to-G editing in human mitochondria. Custom-designed TALEDs were highly efficient in human cells, catalyzing A-to-G conversions at a total of 17 target sites in various mitochondrial genes with editing frequencies of up to 49%.-
dc.language영어-
dc.publisherElsevier B.V.-
dc.titleTargeted A-to-G base editing in human mitochondrial DNA with programmable deaminases-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000800562500001-
dc.identifier.scopusid2-s2.0-85130290051-
dc.identifier.rimsid78256-
dc.contributor.affiliatedAuthorSung-Ik Cho-
dc.contributor.affiliatedAuthorSeonghyun Lee-
dc.contributor.affiliatedAuthorYoung Geun Mok-
dc.contributor.affiliatedAuthorKayeong Lim-
dc.contributor.affiliatedAuthorJaesuk Lee-
dc.contributor.affiliatedAuthorJi Min Lee-
dc.contributor.affiliatedAuthorEugene Chung-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1016/j.cell.2022.03.039-
dc.identifier.bibliographicCitationCell, v.185, no.10, pp.1764 - 1776.e12-
dc.relation.isPartOfCell-
dc.citation.titleCell-
dc.citation.volume185-
dc.citation.number10-
dc.citation.startPage1764-
dc.citation.endPage1776.e12-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaCell Biology-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.subject.keywordPlusMUTATIONS-
dc.subject.keywordPlusELIMINATION-
dc.subject.keywordPlusNUCLEAR-
dc.subject.keywordPlusGENOMIC DNA-
dc.subject.keywordPlusHUMAN-CELLS-
dc.subject.keywordAuthoradenine deaminase-
dc.subject.keywordAuthorbase editing-
dc.subject.keywordAuthorDddAtox-
dc.subject.keywordAuthorDNA-
dc.subject.keywordAuthorgenome editing-
dc.subject.keywordAuthormitochondria-
dc.subject.keywordAuthororganelle-
dc.subject.keywordAuthorTALED-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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