BROWSE

Related Scientist

Lee, Su Ho's photo.

Lee, Su Ho
시냅스 뇌질환 연구단
more info

ITEM VIEW & DOWNLOAD

IRSp53 promotes postsynaptic density formation and actin filament bundling

Cited 0 time in webofscience Cited 0 time in scopus
200 Viewed 0 Downloaded
Title
IRSp53 promotes postsynaptic density formation and actin filament bundling
Author(s)
Feng, Zhe; Suho Lee; Jia, Bowen; Jian, Tao; Eunjoon Kim; Zhang, Mingjie
Publication Date
2022-07
Journal
The Journal of cell biology, v.221, no.8
Publisher
NLM (Medline)
Abstract
IRSp53 (aka BAIAP2) is a scaffold protein that couples membranes with the cytoskeleton in actin-filled protrusions such as filopodia and lamellipodia. The protein is abundantly expressed in excitatory synapses and is essential for synapse development and synaptic plasticity, although with poorly understood mechanisms. Here we show that specific multivalent interactions between IRSp53 and its binding partners PSD-95 or Shank3 drive phase separation of the complexes in solution. IRSp53 can be enriched to the reconstituted excitatory PSD (ePSD) condensates via bridging to the core and deeper layers of ePSD. Overexpression of a mutant defective in the IRSp53/PSD-95 interaction perturbs synaptic enrichment of IRSp53 in mouse cortical neurons. The reconstituted PSD condensates promote bundled actin filament formation both in solution and on membranes, via IRSp53-mediated actin binding and bundling. Overexpression of mutants that perturb IRSp53-actin interaction leads to defects in synaptic maturation of cortical neurons. Together, our studies provide potential mechanistic insights into the physiological roles of IRSp53 in synapse formation and function.
URI
https://pr.ibs.re.kr/handle/8788114/12174
DOI
10.1083/jcb.202105035
ISSN
0021-9525
Appears in Collections:
Center for Synaptic Brain Dysfunctions(시냅스 뇌질환 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse