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Kim, Heejin
식물 노화·수명 연구단
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Posttranscriptional modulation of KCNQ2 gene expression by the miR-106b microRNA family

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Title
Posttranscriptional modulation of KCNQ2 gene expression by the miR-106b microRNA family
Author(s)
Kim, Kwon-Woo; Kim, Keetae; Hee-Jin Kim; Kim, Byeol-I; Baek, Myungin; Suh, Byung-Chang
Publication Date
2021-11-23
Journal
Proceedings of the National Academy of Sciences of the United States of America, v.118, no.47
Publisher
National Academy of Sciences
Abstract
© 2021 National Academy of Sciences. All rights reserved.MicroRNAs (miRNAs) have recently emerged as important regulators of ion channel expression. We show here that select miR-106b family members repress the expression of the KCNQ2 K+ channel protein by binding to the 30-untranslated region of KCNQ2 messenger RNA. During the first few weeks after birth, the expression of miR-106b family members rapidly decreases, whereas KCNQ2 protein level inversely increases. Overexpression of miR-106b mimics resulted in a reduction in KCNQ2 protein levels. Conversely, KCNQ2 levels were up-regulated in neurons transfected with antisense miRNA inhibitors. By constructing more specific and stable forms of miR-106b controlling systems, we further confirmed that overexpression of precursor-miR-106b-5p led to a decrease in KCNQ current density and an increase in firing frequency of hippocampal neurons, while tough decoy miR-106b-5p dramatically increased current density and decreased neuronal excitability. These results unmask a regulatory mechanism of KCNQ2 channel expression in early postnatal development and hint at a role for miR-106b up-regulation in the pathophysiology of epilepsy.
URI
https://pr.ibs.re.kr/handle/8788114/11000
DOI
10.1073/pnas.2110200118
ISSN
0027-8424
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
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