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Kim, Jin Soo
유전체 교정 연구단
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Adenine base editor engineering reduces editing of bystander cytosines

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dc.contributor.authorJeong, You Kyeong-
dc.contributor.authorLee, SeokHoon-
dc.contributor.authorHwang, Gue-Ho-
dc.contributor.authorHong, Sung-Ah-
dc.contributor.authorPark, Se-eun-
dc.contributor.authorJin-Soo Kim-
dc.contributor.authorWoo, Jae-Sung-
dc.contributor.authorBae, Sangsu-
dc.date.accessioned2021-11-29T05:50:00Z-
dc.date.available2021-11-29T05:50:00Z-
dc.date.created2021-08-09-
dc.date.issued2021-11-
dc.identifier.issn1087-0156-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10735-
dc.description.abstractAdenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site. To reduce the cytosine editing activity, we engineered a commonly used adenosine deaminase, TadA7.10, and found that ABE7.10 with a D108Q mutation in TadA7.10 exhibited tenfold reduced cytosine deamination activity. The D108Q mutation also reduces cytosine deamination activity in two recently developed high-activity versions of ABE, ABE8e and ABE8s, and is compatible with V106W, a mutation that reduces off-target RNA editing. ABE7.10 containing a P48R mutation displayed increased cytosine deamination activity and a substantially reduced adenine editing rate, yielding a TC-specific base editing tool for TC-to-TT or TC-to-TG conversions that broadens the utility of base editors. Engineered variants of adenine base editors have reduced cytosine base editing or a specific C-to-G base editing activity.-
dc.language영어-
dc.publisherNATURE RESEARCH-
dc.titleAdenine base editor engineering reduces editing of bystander cytosines-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000668816800001-
dc.identifier.scopusid2-s2.0-85109254847-
dc.identifier.rimsid76174-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1038/s41587-021-00943-2-
dc.identifier.bibliographicCitationNATURE BIOTECHNOLOGY, v.39, no.11, pp.1426 - 1433-
dc.relation.isPartOfNATURE BIOTECHNOLOGY-
dc.citation.titleNATURE BIOTECHNOLOGY-
dc.citation.volume39-
dc.citation.number11-
dc.citation.startPage1426-
dc.citation.endPage1433-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusMUTATIONS-
dc.subject.keywordPlusTARGET-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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