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Lee, Hyun Ji
유전체 교정 연구단
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Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

DC Field Value Language
dc.contributor.authorPark, Soo-Ji-
dc.contributor.authorJeong, Tae Yeong-
dc.contributor.authorShin, Seung Kyun-
dc.contributor.authorYoon, Da Eun-
dc.contributor.authorLim, Soo-Yeon-
dc.contributor.authorKim, Sol Pin-
dc.contributor.authorChoi, Jungmin-
dc.contributor.authorHyunji Lee-
dc.contributor.authorHong, Jeong-Im-
dc.contributor.authorAhn, Jinhee-
dc.contributor.authorSeong, Je Kyung-
dc.contributor.authorKim, Kyoungmi-
dc.date.accessioned2021-11-29T05:30:20Z-
dc.date.available2021-11-29T05:30:20Z-
dc.date.created2021-07-07-
dc.date.issued2021-06-03-
dc.identifier.issn1474-760X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10734-
dc.description.abstract© 2021, The Author(s).Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.-
dc.language영어-
dc.publisherBioMed Central Ltd-
dc.titleTargeted mutagenesis in mouse cells and embryos using an enhanced prime editor-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000660859100002-
dc.identifier.scopusid2-s2.0-85107198784-
dc.identifier.rimsid75973-
dc.contributor.affiliatedAuthorHyunji Lee-
dc.identifier.doi10.1186/s13059-021-02389-w-
dc.identifier.bibliographicCitationGenome Biology, v.22, no.1-
dc.relation.isPartOfGenome Biology-
dc.citation.titleGenome Biology-
dc.citation.volume22-
dc.citation.number1-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaGenetics & Heredity-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryGenetics & Heredity-
dc.subject.keywordPlusGENOMIC DNA-
dc.subject.keywordPlusBASE-
dc.subject.keywordPlusGENERATION-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordAuthorAdamts20-
dc.subject.keywordAuthorChromatin-modulating peptides-
dc.subject.keywordAuthorDwarf phenotype-
dc.subject.keywordAuthorGermline transmission-
dc.subject.keywordAuthorIgf2-
dc.subject.keywordAuthorMouse cells and embryos-
dc.subject.keywordAuthorPrime editor-
dc.subject.keywordAuthorProximal dead sgRNA-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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