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Photoactivatable ribonucleosides mark base-specific RNA-binding sites

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dc.contributor.authorJong Woo Bae-
dc.contributor.authorKim, Sangtae-
dc.contributor.authorV. Narry Kim-
dc.contributor.authorJong-Seo Kim-
dc.date.accessioned2021-11-02T05:30:02Z-
dc.date.available2021-11-02T05:30:02Z-
dc.date.created2021-11-01-
dc.date.issued2021-10-15-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10548-
dc.description.abstractRNA-protein interactions play critical roles in post-transcriptional gene regulation. Here the authors demonstrate pRBS-ID, an updated MS/MS-based method that combines the benefits of photoactivatable ribonucleosides and the chemical cleavage of RNA. RNA-protein interaction can be captured by crosslinking and enrichment followed by tandem mass spectrometry, but it remains challenging to pinpoint RNA-binding sites (RBSs) or provide direct evidence for RNA-binding. To overcome these limitations, we here developed pRBS-ID, by incorporating the benefits of UVA-based photoactivatable ribonucleoside (PAR; 4-thiouridine and 6-thioguanosine) crosslinking and chemical RNA cleavage. pRBS-ID robustly detects peptides crosslinked to PAR adducts, offering direct RNA-binding evidence and identifying RBSs at single amino acid-resolution with base-specificity (U or G). Using pRBS-ID, we could profile uridine-contacting RBSs globally and discover guanosine-contacting RBSs, which allowed us to characterize the base-specific interactions. We also applied the search pipeline to analyze the datasets from UVC-based RBS-ID experiments, altogether offering a comprehensive list of human RBSs with high coverage (3,077 RBSs in 532 proteins in total). pRBS-ID is a widely applicable platform to investigate the molecular basis of posttranscriptional regulation.-
dc.language영어-
dc.publisherNATURE PORTFOLIO-
dc.titlePhotoactivatable ribonucleosides mark base-specific RNA-binding sites-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000707661200006-
dc.identifier.scopusid2-s2.0-85117436782-
dc.identifier.rimsid76621-
dc.contributor.affiliatedAuthorJong Woo Bae-
dc.contributor.affiliatedAuthorV. Narry Kim-
dc.contributor.affiliatedAuthorJong-Seo Kim-
dc.identifier.doi10.1038/s41467-021-26317-5-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, v.12, no.1-
dc.relation.isPartOfNATURE COMMUNICATIONS-
dc.citation.titleNATURE COMMUNICATIONS-
dc.citation.volume12-
dc.citation.number1-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
dc.subject.keywordPlusSPECTROMETRY-
dc.subject.keywordPlusDISCOVERY-
dc.subject.keywordPlusPREDICTION-
dc.subject.keywordPlusCOMPLEXES-
dc.subject.keywordPlusTANDEM MASS-SPECTRA-
dc.subject.keywordPlusCROSS-LINKING-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusIDENTIFICATION-
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Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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