Photoactivatable ribonucleosides mark base-specific RNA-binding sites
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jong Woo Bae | - |
dc.contributor.author | Kim, Sangtae | - |
dc.contributor.author | V. Narry Kim | - |
dc.contributor.author | Jong-Seo Kim | - |
dc.date.accessioned | 2021-11-02T05:30:02Z | - |
dc.date.available | 2021-11-02T05:30:02Z | - |
dc.date.created | 2021-11-01 | - |
dc.date.issued | 2021-10-15 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/10548 | - |
dc.description.abstract | RNA-protein interactions play critical roles in post-transcriptional gene regulation. Here the authors demonstrate pRBS-ID, an updated MS/MS-based method that combines the benefits of photoactivatable ribonucleosides and the chemical cleavage of RNA. RNA-protein interaction can be captured by crosslinking and enrichment followed by tandem mass spectrometry, but it remains challenging to pinpoint RNA-binding sites (RBSs) or provide direct evidence for RNA-binding. To overcome these limitations, we here developed pRBS-ID, by incorporating the benefits of UVA-based photoactivatable ribonucleoside (PAR; 4-thiouridine and 6-thioguanosine) crosslinking and chemical RNA cleavage. pRBS-ID robustly detects peptides crosslinked to PAR adducts, offering direct RNA-binding evidence and identifying RBSs at single amino acid-resolution with base-specificity (U or G). Using pRBS-ID, we could profile uridine-contacting RBSs globally and discover guanosine-contacting RBSs, which allowed us to characterize the base-specific interactions. We also applied the search pipeline to analyze the datasets from UVC-based RBS-ID experiments, altogether offering a comprehensive list of human RBSs with high coverage (3,077 RBSs in 532 proteins in total). pRBS-ID is a widely applicable platform to investigate the molecular basis of posttranscriptional regulation. | - |
dc.language | 영어 | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.title | Photoactivatable ribonucleosides mark base-specific RNA-binding sites | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000707661200006 | - |
dc.identifier.scopusid | 2-s2.0-85117436782 | - |
dc.identifier.rimsid | 76621 | - |
dc.contributor.affiliatedAuthor | Jong Woo Bae | - |
dc.contributor.affiliatedAuthor | V. Narry Kim | - |
dc.contributor.affiliatedAuthor | Jong-Seo Kim | - |
dc.identifier.doi | 10.1038/s41467-021-26317-5 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.12, no.1 | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | SPECTROMETRY | - |
dc.subject.keywordPlus | DISCOVERY | - |
dc.subject.keywordPlus | PREDICTION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | TANDEM MASS-SPECTRA | - |
dc.subject.keywordPlus | CROSS-LINKING | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | IDENTIFICATION | - |