Global and Local Competition between Exogenously Introduced microRNAs and Endogenously Expressed microRNAs
DC Field | Value | Language |
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dc.contributor.author | Doyeon Kim | - |
dc.contributor.author | Jongkyu Kim | - |
dc.contributor.author | Daehyun Baek | - |
dc.date.available | 2015-04-20T05:56:44Z | - |
dc.date.created | 2014-08-11 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/1033 | - |
dc.description.abstract | It has been reported that exogenously introduced micro-RNA (exo-miRNA) competes with endogenously expressed miRNAs (endo-miRNAs) in human cells, resulting in a detectable upregulation of mRNAs with endo-miRNA target sites (TSs). However, the detailed mechanisms of the competition between exo- and endo-miRNAs remain uninvestigated. In this study, using 74 microarrays that monitored the whole-transcriptome response after introducing miRNAs or siRNAs into HeLa cells, we systematically examined the derepression of mRNAs with exoand/or endo-miRNA TSs.We quantitatively assessed the effect of the number of endo-miRNA TSs on the degree of mRNA derepression. As a result, we observed that the number of endo-miRNA TSs was significantly associated with the degree of derepression, supporting that the derepression resulted from the competition between exo- and endo-miRNAs. However, when we examined whether the site proficiency of exomiRNA TSs could also influence mRNA derepression, to our surprise, we discovered a strong positive correlation. Our analysis indicates that site proficiencies of both exoand endo-miRNA TSs are important determinants for the degree of mRNA derepression, implying that the derepression of mRNAs in response to exo-miRNA is more complex than that currently perceived. Our observations may lead to a more complete understanding of the detailed mechanisms of the competition between exo- and endo-miRNAs and to a more accurate prediction of miRNA targets. Our analysis also suggests an interesting hypothesis that long 3-UTRs may function as molecular buffer against gene expression regulation by individual miRNAs. | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.subject | competition, microRNA, miRNA targeting, shRNA, siRNA | - |
dc.title | Global and Local Competition between Exogenously Introduced microRNAs and Endogenously Expressed microRNAs | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000342560600008 | - |
dc.identifier.scopusid | 2-s2.0-84901453808 | - |
dc.identifier.rimsid | 139 | ko |
dc.date.tcdate | 2018-10-01 | - |
dc.contributor.affiliatedAuthor | Doyeon Kim | - |
dc.contributor.affiliatedAuthor | Jongkyu Kim | - |
dc.contributor.affiliatedAuthor | Daehyun Baek | - |
dc.identifier.doi | 10.14348/molcells.2014.0100 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.37, no.5, pp.412 - 417 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 37 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 412 | - |
dc.citation.endPage | 417 | - |
dc.date.scptcdate | 2018-10-01 | - |
dc.description.wostc | 3 | - |
dc.description.scptc | 3 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | TARGETS | - |
dc.subject.keywordPlus | RNAS | - |
dc.subject.keywordAuthor | competition | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | miRNA targeting | - |
dc.subject.keywordAuthor | shRNA | - |
dc.subject.keywordAuthor | siRNA | - |