BROWSE

Related Scientist

csc's photo.

csc
복잡계자기조립연구단
more info

ITEM VIEW & DOWNLOAD

Applicability and Limitations of Fluorescence Intensity-Based Thermometry Using a Palette of Organelle Thermometers

DC Field Value Language
dc.contributor.authorYamazaki, Takeru-
dc.contributor.authorXiao Liu-
dc.contributor.authorYoung-Tae Chang-
dc.contributor.authorArai, Satoshi-
dc.date.accessioned2023-08-08T22:00:41Z-
dc.date.available2023-08-08T22:00:41Z-
dc.date.created2023-08-07-
dc.date.issued2023-07-
dc.identifier.issn2227-9040-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/13717-
dc.description.abstractFluorescence thermometry is a microscopy technique in which a fluorescent temperature sensor records temperature changes as alterations of fluorescence signals. Fluorescence lifetime imaging (FLIM) is a promising method for quantitative analysis of intracellular temperature. Recently, we developed small-molecule thermometers, termed Organelle Thermo Greens, that target various organelles and achieved quantitative temperature mapping using FLIM. Despite its highly quantitative nature, FLIM-based thermometry cannot be used widely due to expensive instrumentation. Here, we investigated the applicability and limitations of fluorescence intensity (FI)-based analysis, which is more commonly used than FLIM-based thermometry. Temperature gradients generated by artificial heat sources and physiological heat produced by brown adipocytes were visualized using FI- and FLIM-based thermometry. By comparing the two thermometry techniques, we examined how the shapes of organelles and cells affect the accuracy of the temperature measurements. Based on the results, we concluded that FI-based thermometry could be used for "qualitative", rather than quantitative, thermometry under the limited condition that the shape change and the dye leakage from the target organelle were not critical.-
dc.language영어-
dc.publisherMDPI-
dc.titleApplicability and Limitations of Fluorescence Intensity-Based Thermometry Using a Palette of Organelle Thermometers-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid001035041800001-
dc.identifier.scopusid2-s2.0-85166279659-
dc.identifier.rimsid81423-
dc.contributor.affiliatedAuthorXiao Liu-
dc.contributor.affiliatedAuthorYoung-Tae Chang-
dc.identifier.doi10.3390/chemosensors11070375-
dc.identifier.bibliographicCitationCHEMOSENSORS, v.11, no.7-
dc.relation.isPartOfCHEMOSENSORS-
dc.citation.titleCHEMOSENSORS-
dc.citation.volume11-
dc.citation.number7-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaElectrochemistry-
dc.relation.journalResearchAreaInstruments & Instrumentation-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalWebOfScienceCategoryElectrochemistry-
dc.relation.journalWebOfScienceCategoryInstruments & Instrumentation-
dc.subject.keywordPlusTEMPERATURE-
dc.subject.keywordPlusVISCOSITY-
dc.subject.keywordAuthorintracellular thermometry-
dc.subject.keywordAuthororganelle thermometer-
dc.subject.keywordAuthorBODIPY rotor-
dc.subject.keywordAuthorFLIM-
dc.subject.keywordAuthorfluorescence-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse