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Off-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing

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Title
Off-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing
Author(s)
Annie Kim; Kun-Gu Lee; Yeongbeen Kwon; Kang-In Lee; Heung-Mo Yang; Omer Habib; Jihun Kim; Sang-Tae Kim; Sung Joo Kim; Jin-Soo Kim; Dong-Youn Hwang
Publication Date
2021-06
Journal
STEM CELL REVIEWS AND REPORTS, v.17, no.3, pp.1053 - 1067
Publisher
SPRINGER
Abstract
Human embryonic stem cells (hESCs) hold promise in regenerative medicine but allogeneic immune rejections caused by highly polymorphic human leukocyte antigens (HLAs) remain a barrier to their clinical applications. Here, we used a CRISPR/Cas9-mediated HLA-editing strategy to generate a variety of HLA homozygous-like hESC lines from pre-established hESC lines. We edited four pre-established HLA-heterozygous hESC lines and created a mini library of 14 HLA-edited hESC lines in which single HLA-A and HLA-B alleles and both HLA-DR alleles are disrupted. The HLA-edited hESC derivatives elicited both low T cell- and low NK cell-mediated immune responses. Our library would cover about 40% of the Asian-Pacific population. We estimate that HLA-editing of only 19 pre-established hESC lines would give rise to 46 different hESC lines to cover 90% of the Asian-Pacific population. This study offers an opportunity to generate an off-the-shelf HLA-compatible hESC bank, available for immune-compatible cell transplantation, without embryo destruction. Graphical
URI
https://pr.ibs.re.kr/handle/8788114/9837
DOI
10.1007/s12015-020-10113-7
ISSN
2629-3269
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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