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Chang, Young Tae
복잡계 자기조립 연구단
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Gynura divaricata Water Extract Presented the Possibility to Enhance Neuronal Regeneration

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Title
Gynura divaricata Water Extract Presented the Possibility to Enhance Neuronal Regeneration
Author(s)
Kantawong, Fahsai; Saisuwan, Chanakarn; Soeratanapant, Pirakorn; Wanachantararak, Phenphichar; Nan, Jiang; Wu, Jianming; Young-Tae Chang
Publication Date
2021-02
Journal
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.2021
Publisher
HINDAWI LTD
Abstract
Gynura divaricata (GD) is an Asian herb widely used as an alternative medicine and functional food for type 2 diabetes. Diabetic neuropathy is considered as an important complication of diabetic patients. This study focused on neuroregenerative effects of GD for use in the prevention of diabetic neuropathy. GD leaves were cut and boiled in water to mimic real-life cooking. The boiled content was filtered through white gauze and lyophilized to preserve as dried powder. Antioxidant assay was performed using DPPH assays. UHPLC-QTOF-MS/MS was employed to test for important compounds in the extract of these herbs. MTT assay was used to test for cell viability. The extracts at concentration of 250 mu g/mL were tested with human gingival cell to observe the change of gene expression. The DPPH assay showed that GD water extract at the concentration of 5000 mu g/mL could inhibit DPPH radical for 39.2%. The results showed that 5000 mu g of GD water extract contained total phenolic content equivalent to 310.9 mu g standard gallic acid. UHPLC-QTOF-MS/MS result found phenolic acids and flavonoids as the main components. Human gingival cells treated with 250 mu g/mL of GD water extract for 10 days showed upregulation of some neuronal differentiation markers. Staining with Cdr3 dye confirmed the presentation of neuronal progenitors. The extract at the concentration of 250 mu g/mL was also tested with apical papilla cells to screen for change of gene expression by RNA sequencing. The result also showed significant upregulation of alpha-internexin (INA). These results indicated that GD water extract might have an inductive effect for neural regeneration and could be used as functional food and supplementation for the prevention or treatment of diabetic neuropathy. This work provided the basic knowledge for further investigations into the benefits of GD for diabetic neuropathy.
URI
https://pr.ibs.re.kr/handle/8788114/9596
DOI
10.1155/2021/8818618
ISSN
1741-427X
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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