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Recording of elapsed time and temporal information about biological events using Cas9

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Title
Recording of elapsed time and temporal information about biological events using Cas9
Author(s)
Jihye Park; Jung Min Lim; Inkyung Jung; Seok-Jae Heo; Jinman Park; Yoojin Chang; Hui Kwon Kim; Dongmin Jung; Ji Hea Yu; Seonwoo Min; Sungroh Yoon; Sung-Rae Cho; Taeyoung Park; Hyongbum Henry Kim
Publication Date
2021-02-18
Journal
CELL, v.184, no.4, pp.1047 - +
Publisher
CELL PRESS
Abstract
DNA has not been utilized to record temporal information, although DNA has been used to record biological information and to compute mathematical problems. Here, we found that indel generation by Cas9 and guide RNA can occur at steady rates, in contrast to typical dynamic biological reactions, and the accumulated indel frequency can be a function of time. By measuring indel frequencies, we developed a method for recording and measuring absolute time periods over hours to weeks in mammalian cells. These time-recordings were conducted in several cell types, with different promoters and delivery vectors for Cas9, and in both cultured cells and cells of living mice. As applications, we recorded the duration of chemical exposure and the lengths of elapsed time since the onset of biological events (e.g., heat exposure and inflammation). We propose that our systems could serve as synthetic "DNA clocks.''
URI
https://pr.ibs.re.kr/handle/8788114/9458
DOI
10.1016/j.cell.2021.01.014
ISSN
0092-8674
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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