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유전체교정연구단
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Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus

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dc.contributor.authorHanh Witte-
dc.contributor.authorEduatdo Moreno-
dc.contributor.authorChristian Rodelsperger-
dc.contributor.authorJungeun Kim-
dc.contributor.authorJin Soo Kim-
dc.contributor.authorAndrian Streit-
dc.contributor.authorRalf J. Sommer-
dc.date.available2015-04-20T05:10:45Z-
dc.date.created2015-01-21-
dc.date.issued2015-01-
dc.identifier.issn0949-944X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/822-
dc.description.abstractThe diplogastrid nematode Pristionchus pacificus is a nematode model system for comparative studies to Caenorhabditis elegans and integrative evolutionary biology aiming for interdisciplinary approaches of evo-devo, population genetics, and ecology. For this, fieldwork can be combined with laboratory studies, and P. pacificus has a welldeveloped methodological toolkit of forward genetics, whole genome sequencing, DNA-mediated transformation, and various –omics platforms. Here, we establish CRISPR/Cas9- based gene inactivation and describe various boundary conditions of this methodology for P. pacificus. Specifically, we demonstrate that most mutations arise within the first 9 hours after injections. We systematically tested the efficiency of sgRNAs targeting different exons in Ppa-dpy-1 and characterized the molecular nature of the induced mutations. Finally, we provide a protocol that might also be useful for researchers working with other non-Caenorhabditis nematodes.-
dc.description.uri1-
dc.language영어-
dc.publisherSPRINGER-
dc.subjectPristionchus pacificus . Nematodes . CRISPR/Cas9 . Gene knockout-
dc.titleGene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000349029300007-
dc.identifier.scopusid2-s2.0-84926669191-
dc.identifier.rimsid16917ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorJin Soo Kim-
dc.identifier.doi10.1007/s00427-014-0486-8-
dc.identifier.bibliographicCitationDEVELOPMENT GENES AND EVOLUTION, v.255, no.1, pp.55 - 62-
dc.citation.titleDEVELOPMENT GENES AND EVOLUTION-
dc.citation.volume255-
dc.citation.number1-
dc.citation.startPage55-
dc.citation.endPage62-
dc.date.scptcdate2018-10-01-
dc.description.wostc35-
dc.description.scptc38-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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