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Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis

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Title
Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis
Author(s)
Muhammad Asri Abdul Sisak; Fiona Louis; Sun Hyeok Lee; Young-Tae Chang; Michiya Matsusaki
Subject
blood capillary models, ; fibrin gels, ; collagen microfibers
Publication Date
2020-08
Journal
MICROMACHINES, v.11, no.8, pp.727
Publisher
MDPI AG
Abstract
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Conventional microarray analysis usually deals with the monolayer or two-dimensional (2D) assays for the high-throughput screening applications. Even though these cell-based assays are effective for preliminary screening at least to have information on cytotoxicity, they do not adequately re-create the in vivo complexity of three-dimensional (3D) tissues. In this study, 3D-blood capillary models were constructed by using physiological collagen microfibers (CMF), which provide the extracellular matrix in the complex tissue. Micro-droplets of fibrin gels containing CMF, endothelial cells, and fibroblasts were cultured for five days in 48-wells plate to provide a medium-throughput system for screening applications. Blood capillaries networks were formed by optimizing the concentration of CMF used and the number of cells. Finally, this screening method was a powerful assay for the application on the selection of not only a specific chemical probe for blood capillary live-imaging, but also a drug, aptamer, and peptide with potential blood vessel targeting property
URI
https://pr.ibs.re.kr/handle/8788114/7673
DOI
10.3390/mi11080727
ISSN
2072-666X
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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