Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9

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Title
Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
Author(s)
Kim, SJ; Omer Habib; Jin-Soo Kim; Han, HW; Koo, SK; Kim, JH
Publication Date
2017-03
Journal
Stem Cell Research, v.19, no., pp.46 - 48
Publisher
ELSEVIER SCIENCE BV
Abstract
Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype. (C) 2016 The Author(s). Published by Elsevier B.V
URI
https://pr.ibs.re.kr/handle/8788114/5802
ISSN
1873-5061
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
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