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유전체교정연구단
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Genome editing reveals a role for OCT4 in human embryogenesis

DC Field Value Language
dc.contributor.authorNorah M. E. Fogarty-
dc.contributor.authorAfshan McCarthy-
dc.contributor.authorKirsten E. Snijders-
dc.contributor.authorBenjamin E. Powell-
dc.contributor.authorNada Kubikova-
dc.contributor.authorPaul Blakeley-
dc.contributor.authorRebecca Lea-
dc.contributor.authorKay Elder-
dc.contributor.authorSissy E. Wamaitha-
dc.contributor.authorDaesik Kim-
dc.contributor.authorValdone Maciulyte-
dc.contributor.authorJens Kleinjung-
dc.contributor.authorJin-Soo Kim-
dc.contributor.authorDagan Wells-
dc.contributor.authorLudovic Vallier-
dc.contributor.authorAlessandro Bertero-
dc.contributor.authorJames M. A. Turner-
dc.contributor.authorKathy K. Niakan-
dc.date.available2017-12-06T05:04:02Z-
dc.date.created2017-11-17-
dc.date.issued2017-10-
dc.identifier.issn0028-0836-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/4009-
dc.description.abstractDespite their fundamental biological and clinical importance, the molecular mechanisms that regulate the first cell fate decisions in the human embryo are not well understood. Here we use CRISPR-Cas9-mediated genome editing to investigate the function of the pluripotency transcription factor OCT4 during human embryogenesis. We identified an efficient OCT4-targeting guide RNA using an inducible human embryonic stem cell-based system and microinjection of mouse zygotes. Using these refined methods, we efficiently and specifically targeted the gene encoding OCT4 (POU5F1) in diploid human zygotes and found that blastocyst development was compromised. Transcriptomics analysis revealed that, in POU5F1-null cells, gene expression was downregulated not only for extra-embryonic trophectoderm genes, such as CDX2, but also for regulators of the pluripotent epiblast, including NANOG. By contrast, Pou5f1-null mouse embryos maintained the expression of orthologous genes, and blastocyst development was established, but maintenance was compromised. We conclude that CRISPR-Cas9-mediated genome editing is a powerful method for investigating gene function in the context of human development. © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleGenome editing reveals a role for OCT4 in human embryogenesis-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000412214100045-
dc.identifier.scopusid2-s2.0-85030775556-
dc.identifier.rimsid60923-
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorJin-Soo Kim-
dc.identifier.doi10.1038/nature24033-
dc.identifier.bibliographicCitationNATURE, v.550, no.7674, pp.67 - 73-
dc.citation.titleNATURE-
dc.citation.volume550-
dc.citation.number7674-
dc.citation.startPage67-
dc.citation.endPage73-
dc.date.scptcdate2018-10-01-
dc.description.wostc38-
dc.description.scptc46-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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