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Highly efficient RNA-guided base editing in mouse embryosHighly Cited Paper
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- Title
- Highly efficient RNA-guided base editing in mouse embryos
- Author(s)
- Kyoungmi Kim; Seuk-Min Ryu; Sang-Tae Kim; Gayoung Baek; Daesik Kim; Kayeong Lim; Eugene Chung; Sunghyun Kim; Jin-Soo Kim
- Subject
- ID HUMAN-CELLS, ; CAS9, ; ENDONUCLEASES, ; MICE, ; SPECIFICITY, ; MUTAGENESIS, ; NICKASES, ; KNOCKOUT
- Publication Date
- 2017-05
- Journal
- NATURE BIOTECHNOLOGY, v.35, no.5, pp.435 - 437
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations. © 2017 Nature America, Inc., part of Springer Nature. All rights reserved
- DOI
- 10.1038/nbt.3816
- ISSN
- 1087-0156
- Appears in Collections:
- Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
- Files in This Item:
- 김경미_nbt.3816.pdfDownload
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