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유전체 교정 연구단
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Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9

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Title
Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9
Author(s)
Gim, Gyeong-Min; Kwon, Dong-Hyeok; Eom, Kyeong-Hyun; Moon, JoonHo; Park, Ji-Hyun; Lee, Won-Wu; Jung, Dae-Jin; Kim, Dae-Hyun; Yi, Jun-Koo; Ha, Jae-Jung; Ka-Yeong Lim; Jin-Soo Kim; Jang, Goo
Publication Date
2022-07
Journal
BIOTECHNOLOGY JOURNAL, v.17, no.7
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Many genome-edited animals have been produced using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology to edit specific genes. However, there are few guidelines for the application of this technique to cattle. The goal of this study was to produce trait-improved cattle using the genome-editing technology CRISPR-Cas9. Myostatin (MSTN) was selected as a target locus, and synthetic mRNA of sgRNA and Cas9 were microinjected into fertilized bovine embryos in vitro. As a result, 17 healthy calves were born, and three of them showed MSTN mutation rates of 10.5%, 45.4%, and 99.9%, respectively. Importantly, the offspring with the 99.9% MSTN mutation rate had a biallelic mutation (-12 bps) and a double-muscling phenotype. In conclusion, we demonstrate that the genome-editing technology CRISPR-Cas9 can produce genetically modified calves with improved traits.
URI
https://pr.ibs.re.kr/handle/8788114/11973
DOI
10.1002/biot.202100198
ISSN
1860-6768
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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