High-throughput in vitro processing of human primary microRNA by the recombinant microprocessor
DC Field | Value | Language |
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dc.contributor.author | kijun Kim | - |
dc.contributor.author | V.Narry Kim | - |
dc.date.accessioned | 2022-03-11T06:30:07Z | - |
dc.date.available | 2022-03-11T06:30:07Z | - |
dc.date.created | 2022-01-25 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/11242 | - |
dc.description.abstract | © 2021 The Author(s)We describe a protocol to conduct a high-throughput in vitro processing assay, using 1,881 human primary microRNAs (pri-miRNAs) and recombinant Microprocessor complex, followed by deep sequencing library generation. This comprehensive approach allows the mapping of cleavage sites and the measurement of processing efficiency of a large number of substrates simultaneously. Our protocol is readily modifiable to investigate the effects of chemicals and regulatory proteins. Moreover, cis-acting elements can be examined by replacing the wild-type pri-miRNAs with mutant variants. For complete details on the use and execution of this profile, please refer to Kim et al. (2021). | - |
dc.language | 영어 | - |
dc.publisher | Cell Press | - |
dc.title | High-throughput in vitro processing of human primary microRNA by the recombinant microprocessor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.scopusid | 2-s2.0-85122238894 | - |
dc.identifier.rimsid | 77144 | - |
dc.contributor.affiliatedAuthor | kijun Kim | - |
dc.contributor.affiliatedAuthor | V.Narry Kim | - |
dc.identifier.doi | 10.1016/j.xpro.2021.101042 | - |
dc.identifier.bibliographicCitation | STAR Protocols, v.3, no.1 | - |
dc.relation.isPartOf | STAR Protocols | - |
dc.citation.title | STAR Protocols | - |
dc.citation.volume | 3 | - |
dc.citation.number | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Bioinformatics | - |
dc.subject.keywordAuthor | Molecular Biology | - |
dc.subject.keywordAuthor | Protein Biochemistry | - |
dc.subject.keywordAuthor | Sequencing | - |