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Impedimetric detection of galactose based on a galactose-binding lectin, Ricinus communis agglutinin I (RCA120)

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Title
Impedimetric detection of galactose based on a galactose-binding lectin, Ricinus communis agglutinin I (RCA120)
Author(s)
Daeho Jeong; Won-Yong Lee
Publication Date
2021-12-15
Journal
Journal of Electroanalytical Chemistry, v.903
Publisher
Elsevier B.V.
Abstract
© 2021 Elsevier B.V.A highly sensitive lectin-based impedimetric detection method has been developed for the determination of galactose in human serum samples. The present method is based on the selective interaction between a target galactose and a galactose-binding lectin, Ricinus communis agglutinin I (RCA120). The quantitative detection of galactose in human serum samples relies on the competition reaction of the target galactose of interest with β-galactose derivative immobilized on a gold electrode surface for a limited amount of RCA120. At equilibrium, the amount of bound RCA120-β-galactose complex on gold electrode surface is inversely proportional to the amount of the target galactose in sample solution. The binding of RCA120 onto the β-galactose-immobilized gold electrode was monitored through electron transfer resistance, which was directly measured by electrochemical impedance spectroscopy. The present method can detect galactose from 8.33 × 10−9 M to 1.25 × 10−6 M (r2 = 0.992) with a limit of detection of 5.87 × 10−9 M (S/N = 3), which is much lower than those obtained with enzyme-based amperometric and colorimetric detection methods. The present method was successfully applied to the determination of galactose in human serum samples.
URI
https://pr.ibs.re.kr/handle/8788114/11070
DOI
10.1016/j.jelechem.2021.115846
ISSN
1572-6657
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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