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Kinetics of the E46Q mutant of photoactive yellow protein investigated by transient grating spectroscopy

      
Title : Kinetics of the E46Q mutant of photoactive yellow protein investigated by transient grating spectroscopy
Issue Date : 201709
Publisher : ELSEVIER SCIENCE BV
Journal : CHEMICAL PHYSICS LETTERS, v.683, no., pp.262 - 267
Abstract : To elucidate the role of internal proton transfer in the photodynamics of photoactive yellow protein (PYP), the photocycle of the Glu46Gln mutant of PYP (E46Q-PYP) is investigated by transient grating (TG) spectroscopy. Compared with wild-type PYP (wt-PYP), the first spectrally blue-shifted intermediate of E46Q-PYP is formed more slowly, which is consistent with the absence of direct protonation from Glu46 residue, if the parallel kinetic model for wt-PYP is invoked. The smaller conformational change in E46Q-PYP, as manifested by the smaller change in the diffusion coefficient, mainly arises from the relatively larger volume of the ground state E46Q-PYP than wt-PYP rather than from the smaller volume of the pB state. (c) 2017 Elsevier B.V. All rights reserved.
ISSN : 0009-2614
Language : English
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